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OJBTM
Online
Journal of Bioinformatics ©
Volume 16(3): 318-326, 2015.
Model simulation phylogenetics
of erythropoietin expressed from cho cell line.
Manoj
Kumar Mettaa, Srinivasan Tantravahib*, Jayasimha
Rayalu Daddamc
a,bDepartment(s)
of Biotechnology, GIS, GITAM University, Gandhi Nagar, Rushikonda,
Visakhapatnam, Andhra Pradesh, cJawaharlal
Nehru Technological University, Anantapur,
Ananthapuram, Andhra Pradesh, India.
ABSTRACT
Mettaa MK, Tantravahib S, Daddam JR., Model
simulation phylogenetics of erythropoietin expressed
from cho cell line, Onl J Bioinform., 16(3): 318-326, 2015. Erythropoietin (EPO), a ~30 kDa glycoprotein essential for erythropoiesis, has a highly
conserved amino acid sequence between monkey and humans (~91% identity), and
other animals. EPO binds and activates EPO receptors (EPO-R) on the surface of erythroid
precursor cells. We modeled a 3D structure
of EPO generated with 1CN4 as template with Modeller7v7 validated with Procheck and Verify 3D graph. After energy minimization, the
3D structure was compared with the template and 8 genes coding for EPO and its loci,
were identified. Multiple alignment and phylogenetic analysis showed that the EPO
family proteins fall into 4 branches. Mutations within the EPO domain region
were identified.
KEY
WORDS: Erythropoietin; Modeling;
Molecular Dynamics; Validation; Phylogenetic analysis.