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OJBTM
Online
Journal of Bioinformatics©
Volume 18(3):119-125, 2017.
Subtractive
genomics for membrane drug targets for antibiotic resistant Chlamydia trachomatis A/HAR-13.
Vijayakumari SM1*, Shivkumar
M1, Biplab B2,Keshava
M2, Jhinuk C2
1Department of Bioinformatics,KSW University, Bijapur, India. 2Department of Biotechnology,
PES Institute of technology, Bangalore, India .
ABSTRACT
Vijayakumari SM, Shivkumar M, Biplab B, Keshava M, Jhinuk C., Subtractive genomics for membrane drug targets
for antibiotic resistant Chlamydia trachomatis A/HAR-13, Onl
J bioinform., 18(3):119-125, 2017. A subtractive
genomics approach was employed to identify metabolic pathways-related candidate
drug and vaccine targets in Chlamydia
trachomatis virulent strain A/HAR-13. A filter for drug proteome based on pre-set
conditions to generate a set of membrane associated human-non homologous drug targets was used. Survival
and segregation of Chlamydia trachomatis
unique metabolic pathway proteins and structure generation of the membrane
associated drug targets was validated. Five membrane associated drug targets participating
in peptidoglycan biosynthesis; cell cycle caulobacter
& lipopolysaccharide biosynthesis were identified and their 3D structures
generated & validated. These putative drug targets identified can be used as
a platform for screening against a battery of anti-microbial leads. Computational analysis of the result
indicates that out of 919 proteins of Chlamydia
trachomatis A/HAR-13; 314 were found to be essential after subjecting the
non-homologous sequence to blastp against the DEG
database. The essential gene of Chlamydia
trachomatisA/HAR-13 is listed and analyzed for cellular
localization and biochemical metabolic pathway analysis. The list of proteins
predicted to have unique pathway is given and drug targets having unique
pathogenic specific pathways localized in membrane were considered for further
analysis since membrane bound proteins are easier to target and as they are
surface proteins they are exposed to immune system as can be used an epitopes
for vaccine design. A list of putative drug targets with its predicted
function, localization, and pathway is presented.
Keywords:
subtractive genomics, drug targets, Chlamydia
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