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OJBTM
Online Journal of
Bioinformatics ©
Volume 16(3): 303-317, 2015.
In silico
analysis of arsenate reductase gene in biological systems
Sarita Tiwari1, Bijaya Ketan Sarangi1 Jeya Nasim2, Dinesh Yadav2
1National Environmental Engineering Research Institute, Council of
Scientific and Industrial Research, Nehru Marg, Nagpur 2Department of Biotechnology, DDU Gorakhpur University, Gorakhpur, Uttar
Pradesh, India.
ABSTRACT
Tiwari S, Sarangi BK, Nasim
J, Yadav D., In silico analysis of arsenate reductase gene in biological
systems, Onl J Bioinform., 16(3): 303-317, 2015. ArsC cistron operon reduces arsenate As(V) to arsenite As(III), a
primary step in arsenic (As) detoxification. It is presumed that this mechanism
has evolved among prokaryotic bacteria and eukaryotic yeast and plants. 174 arsenate
reductase (AR) protein sequences from 5 different species retrieved from NCBI
were analyzed for similarity, evolution pathway, homology search, multiple
sequence alignment, phylogenetic tree construction, motif and functional
divergence. Conserved regions at different stretches of the sequences revealed
the identity of AR and the phylogenetic tree showed 5 major cluster source
organisms Escherichia coli, Staphylococcus aureus, Saccharomyces
cerevisiae, Arabidopsis thaliana and Pteris
vittata. Variability in the distribution of the
six motifs was observed along with unique motif 4 and motif 6 exclusively for S.
aureus and P. vittata respectively. The coefficient of
functional divergence was found to be <1, indicating prevalence of site-specific
selective constraints leading to functional evolution after diversification.
Keywords: Arsenic, arsenate reductase, evolution,
phylogenetic relation