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Online Journal of Veterinary Research©
Volume 24 (5):281-283, 2020.
Effect of zinc on Trichomonas vaginalis and antioxidant enzymes in Vitro.
Maral Payedar1, Seyed Mostafa Razavi1 , Saeed Nazifi2
1Department(s) of Pathobiology, 2Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Payedar M, Razavi SM, Nazifi S., Effect of zinc on Trichomonas vaginalis and antioxidant enzymes in Vitro, Onl J Vet Res., Volume 24 (5):281-283, 2020. Trichomonas vaginalis is a common venereal infection often unsuccessfully treated with metronidazole. We report growth and antioxidants in T. vaginalis cultured in Trypticase -Yeast Extract-Maltose media with or without 100, 150, and 200 µg zinc/ml. Number of parasites and glutathione peroxidase (GPX), superoxide dismutase (SOD) and catalase (CAT), were determined 8 times 3 to 10 days post culture. Compared with controls, in 100 µg/ml zinc enzymes increased (P < 0.05) 21-25%, parasites 4.5 fold, and protein 17%. At 150µg/ml, zinc had no effect on parasites or enzymes but at 200µg/ml it appeared to eradicate T. vaginalis. We also found a correlation (P <0.05; r = 0.72 between T. vaginalis and protein. We conclude that at lower doses (100µg/ml) actually enhances T vaginalis. We surmise that unsuccessful treatment with metronidazole in patients given zinc supplementation may be due to higher antioxidant resistance to free radicals and not drug resistance per se.
Keywords: Zinc, T. vaginalis, metronidazole, antioxidant enzymes.