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Online Journal of Veterinary Research©
Volume 23 (3):206-213, 2019.
Effect of zinc oxide nanoparticles on sperm cell comet assay, testis malondialdehyde
and glutathione peroxidase levels in adult rats.
Wassan M Husain1, Orooba MS Ibrahim*2, Jawad K Araak2
Al- Yarmouk University College1, College of Veterinary Medicine -Baghdad University2
Husain WM, Ibrahim OMS, Araak JK., Effect of zinc oxide nanoparticles on sperm cell comet assay, testis malondialdehyde and glutathione peroxidase levels in adult rats., Onl J Vet Res., 23 (3):206-213, 2019. Groups of 6 adult Wistar rats were gavaged 5, 10, 20 and 40 mg/kg daily zinc oxide nanoparticles (ZnNano) daily for 56 days. By day 56, rats were anaesthetized for comet assay of sperm cells, and testes homogenized for malondialdehyde (MDA) and glutathione peroxidase (GPX). Compared with water controls we found dose dependent rises (P<0.05) in MDA (35-116%) and declines (22-60%) in glutathione peroxidase in testis tissue. In those without comets, decreased ~23% in rats given 20-40mg/kg zinc nanoparticles and in those with high number of comets increased twofold ~220%. In rats given >10mg/kg comet lengths increased ~45-168%, tail lengths about ten-fold, DNA in tail about 5000 fold, and tail and olive moments about 3000 fold. We found no effect on head DNA. Our findings suggest that doses greater than 5mg/kg zinc nanoparticles given chronically (56 Days or more may adversely affect testes of rats.
Key words: Zinc, Nanoparticle, Sperm, Genotoxicity, Malondialdehyde, glutathione peroxidase.