1996-2019. All Rights Reserved. Online Journal of Veterinary Research. You may not store
these pages in any form except for your own personal use. All other usage or
distribution is illegal under international copyright treaties. Permission
to use any of these pages in any other way besides the before mentioned must be
gained in writing from the publisher. This article is
exclusively copyrighted in its entirety to OJVR. This article may be copied
once but may not be, reproduced or re-transmitted without the express
permission of the editors. This journal satisfies the refereeing requirements
(DEST) for the Higher Education Research Data Collection (Australia). Linking:
To link to this page or any pages linking to this page you must link directly
to this page only here rather than put up your own page
OJVRTM
Online Journal of Veterinary Research©
Volume
21(4):173-177, 2017.
Morphological and functional
characteristics of pancreas in adult Zebrafish Danio rerio over 12 weeks
Kaique Nogueira, Anna C. Santos, Andre R. C.
Barreto-Vianna, Carolina S. Leite, Marcio B. Castro, Eduardo M. M. Lima.
Laboratory of Anatomy of Domestic Animals, Faculty of Agronomy and Veterinary Medicine, Brasilia, DF, Brazil
ABSTRACT
Nogueira K, Santos AC, Barreto-Vianna
ARC, Leite CS, Castro MB, Lima EMM., Morphological
and functional characteristics of pancreas in adult Zebrafish Danio rerio over 12 weeks, Onl J Vet
Res., 21(4):173-177, 2017. Morphological and stereometric functional changes over time in Zebrafish
pancreas are described. Thirty-six
zebrafish of 12 groups of 3 adult fish
each representing a study week were used. At the end
of each week fish were euthanized, pancreas excised and fixed for microphotographic measurement. Graphs of 3 random fields of
tissue from each fish/week were used to determine volume density (Vv) of pancreatic acini and islets, adipocytes, and vessels by histologic score and the Delesse principle. Density
of acini were 57 ± 11% at 2 weeks compared with 79 ± 10% at 4 weeks, islets ~6.9 ± 6.4% at 7 weeks versus 16.1 ± 17.6% at 1 week and
vessels 2.2 ± 3.9% at 5 weeks and 7.4% ± 7.3 at 2 weeks, adipocytes 4.9
± 6.3% at week 1 and 32.4 ± 15.8% at 2 weeks age. Density of acini were
significantly different between 1st and 2nd, 2nd
and 4th, 2nd and 5th, 2nd and 8th,
and 2nd and 10th weeks but the was no difference in
density of islets or vessels at any age. Density of adipocytes was
significantly different between the 1st and 2nd, 2nd and 4th,
and 2nd and 10th weeks. No significant correlation was
found between volume density and age but there was a highly significant
negative correlation between densities of acini with adipocytes. Volume
densities of the pancreas of adult zebrafish showed no significant
morphological changes over 12 weeks. Further studies to evaluate other
pancreatic structures such as connective tissue and different types of collagen
present should be pursued.
KEY WORDS: Danio rerio;
Quantification; Fish; Anatomy; Gland.