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Online Journal of Veterinary Research©
Volume 25(1): 39-46, 2021
Effect of Withania somnifera on HepG2 cell mercury induced toxicity.
Vivek Singh, Anu Rahal, KP Singh and AH Ahmad
Department of Pharmacology and Toxicology,College of Veterinary & Animal Sciences,G.B.Pant University of Agriculture & Technology, Pantnagar India
Singh V, Rahal A, Singh KP, Ahmad AH., Effect of Withania somnifera on HepG2 cell mercury induced toxicity, Onl J Vet Res., 25(1): 39-46, 2021. We report in vitro effect of withania somnifera extracts on mercury toxicity in Hep G2 cell line. MTT cell viability assay showed that mercury produced dose dependent cytotoxic IC50 of 36.41±1.18 μM after 12 h of exposure. We found increased (P < 0.05) viability in cells pre-incubated with extract or incubated simultaneously with mercury. Compared with 25μM mercury controls, 64.66±1.39% viability increased to 66.01± 2.95% in 2.5, 67.40±3.66 in 5, 70.85±3.82 in 7.5 and 72.30±2.96 % in 10uM mercury but 65.91±3.04, 68.01±3.14, 73.44±4.61 and 74.37±3.77 % when cells were treated with Withania extract 6h before challenge with mercury chloride. Similarly in cells treated with 50μM HgCl2 viability was 36.80±1.87 % which increased to 38.41±1.84, 39.37±1.03, 40.60±1.91 and 44.25±1.91 % in 2.5, 5, 7.5 and 10 μg/ml Withania extract given simultaneously or 39.08±2.41, 40.23±1.15, 42.33±1.90 and 46.46±2.42% when cells were treated with extract 6h before challenge of mercury chloride. We find that W. somnifera root extract administered 6h prior or simultaneously with mercury chloride challenge may increase Hep G2 cell viability.
Keywords: Withania: Hep G2; mercury; cell viability.