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OJVRTM
Online Journal of Veterinary Research©
(Including Medical and Laboratory
Research)
Established 1994
ISSN 1328-925X
Volume
25 (10):719-728, 2021.
Effects of Juglans nigra walnut diet on serum melatonin and amygdala
kindling epilepsy in male Wistar rats.
Shaahin Harandi (MSc)1,2,
Mehdi Abbasnejad (PhD)1, Javad Mirnajafi-Zadeh (PhD)3,Saeed
Esmaeili-Mahani (PhD)1,Khadije Esmaeilpour (MSc)2,
Majid
Asadi-Shekaari (PhD)2,
Ali Mostafavi (PhD)4, Vahid
Sheibani (PhD)2*
Department(s) 1Biology,
Faculty of Sciences, Shahid Bahonar
University of Kerman, 2Neuroscience Research Center, Kerman
University of Medical Sciences, 3Physiology, Faculty of Medical
Sciences, Tarbiat Modares
University, Tehran, 4Chemistry, Shahid Bahonar, University of Kerman, Kerman, Iran.
ABSTRACT
Harandi S, Abbasnejad M, Mirnajafi-Zadeh
J, Esmaeili-Mahani S, Esmaeilpour
K, Asadi-Shekaari M, Mostafavi
A, Sheibani V., Effects of Juglans nigra
walnut diet on serum melatonin and amygdala kindling epilepsy in male Wistar rats, Onl J Vet Res., 25 (10):719-728, 2021. We report effect of a
walnut diet and melatonin on amygdala kindling model of epilepsy (ADD) in male Wistar rats. Three groups of six 250-300g rats each were
fed 2 months 1.2g walnut daily, injected 4.5ng melatonin and saline twice
weekly before ADD stimulations. Six controls were fed standard diet and then
stimulated as above. Bipolar stimulating and monopolar electrodes were placed
in right basolateral amygdala of all rats. Compared with control or saline
rats, we found no ADD differences until day 8 when seizures declined (P <
0.05)~70% in rats fed walnuts and 84% in those
injected melatonin and by day 14, 36% and 56%, respectively. These differences
persisted day 7 to 14. ADD stimulations to reach stages 3 to 5 required ~40 to
65% more in rats given walnuts and melatonin and 3 to 5 fold during the first
13 days. We assayed 3.79 ± 0.12ng melatonin/g walnuts and in rat serum injected
melatonin 201 ± 6.3ng/L and controls 168.1 ± 7.8 ng/L.
Results suggest that walnuts may exert anti-epileptic effect possibly by
increasing circulating melatonin.
Keywords: Amygdala; Epilepsy;
Kindling; Melatonin; Seizure; Juglandaceae (Walnut).
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