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Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X


 Volume 25 (10):719-728, 2021.

Effects of Juglans nigra walnut diet on serum melatonin and amygdala kindling epilepsy in male Wistar rats.


Shaahin Harandi (MSc)1,2, Mehdi Abbasnejad (PhD)1, Javad Mirnajafi-Zadeh (PhD)3,Saeed Esmaeili-Mahani (PhD)1,Khadije Esmaeilpour (MSc)2,

Majid Asadi-Shekaari (PhD)2, Ali Mostafavi (PhD)4, Vahid Sheibani (PhD)2*


Department(s) 1Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, 2Neuroscience Research Center, Kerman University of Medical Sciences, 3Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, 4Chemistry, Shahid Bahonar, University of Kerman, Kerman, Iran.




Harandi S, Abbasnejad M, Mirnajafi-Zadeh J, Esmaeili-Mahani S, Esmaeilpour K, Asadi-Shekaari M, Mostafavi A, Sheibani V., Effects of Juglans nigra walnut diet on serum melatonin and amygdala kindling epilepsy in male Wistar rats, Onl J Vet Res., 25 (10):719-728, 2021. We report effect of a walnut diet and melatonin on amygdala kindling model of epilepsy (ADD) in male Wistar rats. Three groups of six 250-300g rats each were fed 2 months 1.2g walnut daily, injected 4.5ng melatonin and saline twice weekly before ADD stimulations. Six controls were fed standard diet and then stimulated as above. Bipolar stimulating and monopolar electrodes were placed in right basolateral amygdala of all rats. Compared with control or saline rats, we found no ADD differences until day 8 when seizures declined (P < 0.05)~70% in rats fed walnuts and 84% in those injected melatonin and by day 14, 36% and 56%, respectively. These differences persisted day 7 to 14. ADD stimulations to reach stages 3 to 5 required ~40 to 65% more in rats given walnuts and melatonin and 3 to 5 fold during the first 13 days. We assayed 3.79 0.12ng melatonin/g walnuts and in rat serum injected melatonin 201 6.3ng/L and controls 168.1 7.8 ng/L. Results suggest that walnuts may exert anti-epileptic effect possibly by increasing circulating melatonin.


Keywords: Amygdala; Epilepsy; Kindling; Melatonin; Seizure; Juglandaceae (Walnut).