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Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X


Volume 28(6): 327-338, 2024.

Effect of vitamin C on gentamicin-induced nephrotoxicity in albino rats.


Amin Derakhshanfar, Arash Bidadkosh, Ali Roshanzamir.


Department of Pathology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.




Derakhshanfar A, Bidadkosh A, Roshanzamir A., Effect of vitamin C on gentamicin-induced nephrotoxicity in albino rats, Onl J Vet Res., 28(6): 327-338, 2024. Groups of 5 Albino rats were injected IM daily for 10 days 80 mg/kg gentamicin, with 100 or 200mg/kg vitamin C. Controls were injected saline. Gentamicin increased (P < 0.05) plasma MDA ~55% but reduced creatinine clearance ~89%, serum sodium ~61%, potassium 2% and cytoplasmic superoxide dismutase ~4% compared with controls. Higher doses of vitamin C (200 mg/kg/body wt) with gentamicin decreased creatinine clearance ~32%, plasma MDA 37% and serum sodium ~21%. We found higher doses of vitamic C reduced MDA further (P < 0.05) compared with lower doses. Microscopy revealed severe acute tubular necrosis in animals given gentamycin per se which appeared to be ameliorated by vitamic C. Gentamicin-treated animals at a dose of 80 mg/kg for 6 days demonstrated a typical pattern of nephrotoxicity characterized by increased serum creatinine and necrosis of proximal tubular epithelium. Findings suggest that high dose of vitamin C may inhibit functional and histological renal changes induced by gentamicin in Albino rats.


Keywords: Gentamicin, vitamin C, nephrotoxicity, rat, superoxide dismutase, peroxidation.