1994-2019. All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors.


OJVRTM

 Online Journal of Veterinary Research

Volume 22 (7):587-595, 2018.


Effect of vitamin D on reproductive hormones, ovarian and uterine histology in Wistar rats gavaged aluminum chloride.

 

Shatha Q. Jaber and Tahani S.S. Al-Azawi PhD.

 

Department of Physiology & Pharmacology, College of Veterinary Medicine, University of Baghdad-Iraq.

 

ABSTRACT

 

Jaber SQ, Al-Azawi TSS., Effect of vitamin D on reproductive hormones, ovarian and uterine histology in Wistar rats gavaged aluminum chloride, Onl J Vet Res., (7):587-595, 2018. Groups of 5 Wistar rats each were gavaged 5000 IU vitamin D (VD) or 200mg/kg aluminum chloride (AL) daily for 15 days. Two other groups were gavaged VD for 15 days and then AL, for a further 15 days, or vice versa. Controls were given saline. Blood was taken at 15 and 30 days for determination of serum follicular stimulating, (LSH), luteinizing (LH), estradiol (E) and progesterone (P). At necropsy, ovaries and uterus were excised for microscopy and index values. Compared with controls (P < 0.05), serum FSH and progesterone were higher in rats given VD or VD before AL, LH and E were lower in all groups, ovarian index higher in all, and uterine index higher in those given VD. Compared with control microscopy, rats given vitamin D had prominent corpus luteum and aggregation of polymorphic nuclear cells on uterine stroma and adjacent glands. In rats given only aluminum chloride, we observed severe degenerative changes in primordial follicles and hemorrhage with congestion of the endometrium. Our results suggest that vitamin D may protect and/or induce regeneration of ovarian follicles before administration of aluminum chloride in Wistar rats.

 

Key words: vitamin D, Aluminum chloride, Female rats, reproductive system.