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Online Journal of Veterinary Research©
Volume 22 (7):571-577, 2018.
Effect of oral challenge with virulent E.coli O157:H7 on clinical signs and histopathology in lambs.
Ali Hussein fadhil1; Afaf Abdulrahman Yousif2
1Dept. of Internal and Preventive Vet. Medicine/ College of Vet. Medicine/ University of Kerbala, Iraq. PhD. 2Dept. of Internal and Preventive Vet. Medicine/ College of Vet. Medicine/ University of Baghdad, Iraq.
Fadhil AH, Yousif AA., Effect of oral challenge with virulent E.coli O157:H7 on clinical signs and histopathology in lambs, Onl J Vet Res., 22 (7):571-577, 2018. Authors report effect of single oral challenge with virulent E.coli O157:H7 on clinical signs and histopathology in lambs. Groups of five 2-6 month old Awassi lambs each were challenged orally 2 ml of 1X1010 cell CFU/ml virulent E.coli O157:H7 and monitored for clinical signs, histopathology and mortality. Controls were injected 2ml PBS subcutaneously. Lambs suffered anorexia, diarrhea and dehydration and one died 6 days post challenge. Microscopy of large intestine revealed neutrophil, macrophage and lymphocyte infiltration in mucosa and submucosa with destruction of epithelial layer in the large intestine. Liver slides showed mononuclear cells aggregation in the portal area with necrosis of hepatocytes. In kidneys we found acute cellular degeneration of epithelial cells of dilated renal tubules.
Key Words: E.coli O157:H7 bacteria, lambs, challenge study, Kidney, Large intestine.