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Online Journal of Veterinary Research


Volume 26 (7): 546-557, 2022.

Effect of n-methyl-n-nitroso urea on vascular endothelial growth factor mRNA in spleen of rats

Sajjarattul NNA BSc, Hazilawati H DVM MVSc PhD1, Rosly SM DVM MSc2, Hakimi MK BSc MSc, Nursyuhada H BSc MSc, Yusnani MY BSc MSc,

Hutheyfa AH DVM MVSc, Nurul Huda MZ DVM, Shanmugavelu S DVM MPhil PhD2, Noordin MM DVM MSc PhD1, Jasni S DVM PhD3, Tan SW BSc PhD4


1Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, 2Strategic Livestock Research Centre, Malaysian Agricultural Research and Development Institute, Serdang, 3Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kampus Kota, Kota Bharu,4Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.




Sajjarattul NNA, Hazilawati H, Rosly SM, Hakimi MK, Nursyuhada H, Yusnani MY, Hutheyfa AH, Nurul Huda MZ, Shanmugavelu S, Noordin MM, Jasni S, Tan SW, Effect of n-methyl-n-nitroso urea on vascular endothelial growth factor mRNA in spleen of rats, Onl J Vet Res., 26 (7): 546-557, 2022. Vascular endothelial growth factor (VEGF) gene induces angiogenesis. VGEF expression and its receptors have been found in lymphoma cells. We report transcription of VEGF in spleen of female Sprague Dawley rats given N-methyl-N-nitroso-urea (MNU). Groups of nine 6 week old rats each were gavaged 60mg/kg (1) or injected intraperitoneal MNU (2) 4 times over 14 days. Controls consisted of six rats. Spleens were excised from euthanized at 24 weeks for RNA extraction and transcription of VEGF by qRT-PCR. RNA was converted to cDNA followed by amplification by RT-PCR. Amplification of VEGF transcripts were normalized with housekeeping genes glyceraldehydes-3-phosphate dehydrogenase (GAPDH) and beta-actin. Relative transcription of VEGF in spleen of rats injected MNU was 360.3823.25, which was 316 times higher (P < 0.05) than controls (1.140.08), whereas in those gavaged MNU, transcription was 44.971.07 or 39 times higher compared with controls. Results suggested that VEGF was highly transcribed when MNU gavaged.


Keywords: angiogenesis, splenic lymphoma, real time RT-PCR assay.