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Online Journal of Veterinary Research


Volume 26 (5): 357-364, 2022.

In vitro antibacterial activity of Vespa orientalis wasp venom.


Jalaei J MS PhD, Fazeli M DVM PhD, Rajaian H DVM PhD, Shomali T DVM PhD.


Division of Pharmacology and Toxicology, Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.



Jalaei J, Fazeli M, Rajaian H, Shomali T., In vitro antibacterial activity of Vespa orientalis wasp venom, Onl J Vet Res., 26 (5): 357-364, 2022. Venom Vespa orientalis wasp sac extract minimum inhibitory concentration (MIC) against Staphylococcus intermedius, Bacillus cereus, Pseudomonas aeruginosa and Salmonella typhimurium was determined by microtiter broth dilution and disk diffusion. Tetracycline was used as the positive control. Control curves were compared with organisms grown in broth with 1 and 2 times MIC or 16 g/ml tetracycline for 24 h. P. aeruginosa was most resistant (50%) and S. typhimurium most sensitive to venom by MIC. The antimicrobial activity of Vo venom against all tested bacteria was concentration-dependent as demonstrated by growth curves. However compared with tetracycline control ~32-128 times more vespa orientalis was required for equivalent inhibition. In conclusion, Vo venom shows in vitro antibacterial activity against S. intermedius, B. cereus, P.aeruginosa and S. typhimurium, although its MIC values were much higher than tetracycline.


Key words: Vespa orientalis venom; antibacterial effect; in vitro