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OJVRTM
Online Journal of Veterinary Research©
Volume
18(2):151-166, 2014. Redacted 2017.
Adaptation and attenuation of fowl adenovirus in Vero cells
Norfitriah MS1 (DVM),
Hair-Bejo M1,2 (DVM, Ph.D), Omar AR1,2 (DVM, Ph.D), Aini
I1,2 (DVM, Ph.D) and Nurulfiza MI2,3 (BS, Ph.D)
1Faculty of Veterinary Medicine, 2Institute of Bioscience, 3Faculty
of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400
UPM Serdang, Selangor, Malaysia Corresponding Author: Hair-Bejo M, Faculty of
Veterinary Medicine, Universiti Putra
Malaysia, 43400 UPM Serdang, Selangor, Malaysia
ABSTRACT
Norfitriah MS, Hair-Bejo M, Omar AR, Aini I, Nurulfiza MI., Adaptation
and attenuation of fowl adenovirus in Vero cells, Onl J Vet Res., 18(2):151-166,
2014. Highly pathogenic Fowl
Adenoviruses (FAdVs) have been recognised as primary pathogens of inclusion
body hepatitis (IBH) in many poultry producing areas, and thus there is a need
to develop a vaccine for its control and prevention. Adaptation and attenuation
of FAdV (UPM1137) isolated from chickens with IBH in Vero cells is described.
Confluent monolayer of Vero cells was inoculated with 0.1mL FAdV inoculum and
monitored daily for 7 days for cytopathic effects. Cytopathic effects were
recorded on day 6 from the 1st to 5th passage. Cytopathic damage was induced by
FAdV as shown by PCR and sequence analysis. The virus adapted and attenuated up
to passage 5 in the presence of nucleotide and amino acid changes in the L2
loop variable region. Amino acids changes occurred at position 506, 512 and
regions between 513 to 514, 521 and 530 (numbering with reference to HAV-2).
Phylogenetic trees revealed all samples from the original homogenate embryo
liver (P0), first passage (P1) and fifth passage (P5) infected Vero cells were
classified under FAdV group E strain.
Key words: Fowl
Adenovirus (FAdVs), Vero cells, adaptation, attenuation, cytopathic effect
(CPE), polymerase chain reaction (PCR)
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