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OJVRTM
Online
Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 27 (12): 659-663, 2023.
Providencia vermicola isolated by RT-PCR and
antibiotic resistance associated with diarrhea in cattle.
Nagham M. Al‐Gburi
Zoonotic Diseases Unite, College of Veterinary
Medicine, Baghdad University.
ABSTRACT
Al‐Gburi NM., Providencia
vermicola isolated by RT-PCR and antibiotic
resistance associated with diarrhea in cattle,
Onl J Vet Res., 27 (12) : 659-663, 2023. Sixty fecal samples were collected from cattle exhibiting
severe diarrhea. Samples were inoculate
into pepton water, incubated 24h at 37°C, subcultured on McConkey blood
agars 24h at 37 °C. By microscopy, culture and biochemical tests we identified Providencia vermicola
confirmed by PCR 16S rRNA gene with 100% homology.
The isolate was tested for susceptibility to 10 antimicrobials by disk
diffusion and found to be resistant to 5 of them. BAB812 strain was highly
resistant to trimethoprim with sulfamethoxazole, polymyxin
B, trimethoprim, tetracycline, neomycin and sulfafurazole
whereas P.vermicola BAB813 strain exhibited high
resistance to chloramphenicol, polymyxin B and
tetracycline.
Keywords: cattle, P.vermicola,
diarrhea,Iraq, Providencia,Antibiotic resistance test.
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