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OJVRTM
Online
Journal of Veterinary Research©
Volume 25(2): 154-162, 2021.
Effect of single and
combined high and low dose verapamil and colchicine on
behavior, cholinesterase and creatinine kinase in Webster albino
mice.
Tayseer Shaker Mahmood1, Duraid Abdul-Hadi Abass2
1Nursing Department, Al-Hadi University College,2Department of Physiology, Biochemistry & Pharmacology, College of Veterinary
Medicine, University of Baghdad, Iraq.
ABSTRACT
Mahmood TS, Abdul-Hadi Abass D., Effect of single and combined high and low dose
verapamil and colchicine on behavior, cholinesterase and creatinine kinase in
Webster albino mice, Onl J Vet Res., 25(2): 154-162, 2021. P-glycoprotein inhibitors verapamil and
colchicine interactions may affect behaviour and cholinesterase activity in
mice. Groups of 7 Webster albino mice each were gavaged
50 and 70 µg/kg colchicine with or without 1.5 and 3mg/kg verapamil daily for
90 days. Controls were gavaged distilled water. To
evaluate behavior, 3 minute open field crossings, geotaxis, cliff, Y-maze, swim
and head pocking tests were performed at days 0, 45 and 90. We determined serum
acetylcholine and creatinine kinase at days 45 and 90. Compared with Day 0 and
water controls, by day 90, colchicine and verapamil open crossings declined ~-19-36%
(P < 0.05) but combined had no effect. At Day 45, negative geotaxis was not
affected but increased 61-75% by day 90. Cliff avoidance increased 32-77% with
single drugs but only 47-52% when combined. Head pocking declined 25-63% with all
treatments and 12 Y maze trials by 19-42%, but combined had no effect. We noted
that 20 second swimming declined ~25% in all groups. Cholinesterase increased
~33% in mice given colchicine but declined ~15-60% with other treatments. Serum
creatinine kinase declined 23-63% in mice given verapamil or combined drugs but
colchicine had no effect. We found time based drug dose dependency and results
suggested that higher single and combined doses had most effect on locomotor,
memory and mentation.
Keywords: P-glycoprotein,
colchicine, verapamil, behavior, cholinesterase, Creatine.