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Online Journal of Veterinary Research©
Volume 22 (6):473-485, 2018.
Effects of aqueous extract of Adiantum capillus-veneris on hematology,
blood glucose and liver tissue in diabetic BALB/C male mice
Mohammad Mahdi Zangeneh1,2*, Sahar Hozhabri3
1Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, 2Pharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, 3Department of Pharmaceutical Chemistry, Zagros University, Kermanshah, Iran. *Corresponding Author: Mohammad Mahdi Zangeneh. *Email: Zangeneh.email@example.com
Zangeneh MM, Hozhabri S., Effects of aqueous extract of Adiantum capillus-veneris on hematology, blood glucose and liver tissue in diabetic BALB/C male mice, Onl J Vet Res., 22 (6):473-485, 2018. Effects of Adiantum capillus-veneris aqueous extract (ACAE) on hematology, serum enzymes and hepatic markers in Streptozotocin (STZ)-induced diabetic BALB/C male mice are described. Six groups of 10 adult male mice each were injected 60 mg/kg STZ IP to induce diabetes. Two groups of 10 served as non-diabetic and untreated diabetic controls and one group of 10 as a glibenclamide (20mg/kg) standard. Diabetic mice with fasting blood glucose (FBG) >250 mg/dL were then gavaged orally 20 mg/kg glibenclamide and gavaged orally 10, 20, 40 or 80 ACAE mg/kg daily for 20 days. Tail venous Blood samples were taken at 0, 7, 13 and 20 days after inducing diabetes. At the 20th day, euthanized mice were dissected for hematology, serum enzymes and liver pathology. FBG in diabetic controls remained at ~490mg/dl throughout whereas in mice given 10mg/kg or more ACAE, declined (p ≤ 0.05) after 7 to 20 days together with glibenclamide standards. Compared with saline controls ALT, AST and ALP increased (p ≤ 0.05) and RBC, MCV, Hb, MCH and MCHC and PCV declined (p ≤ 0.05) in diabetic controls. In mice given 40 or 80mg/dl ACAE there was no difference with hematology compared with saline controls. Hemoglobin remained normal in all groups except diabetic controls where it declined (p ≤ 0.05). Treatment with 80mg/dl ACAE prevented increases (p ≤ 0.05) in platelets compared with diabetic controls. Compared with saline controls, WBC, eosinophils and basophils (p ≤ 0.05) increased but lymphocytes and monocytes declined (p ≤ 0.05) in diabetic controls. Treatment with ACAE prevented these changes in WBC. We found no change in neutrophils in any group. Compared with saline controls, SOD and CAT fell (p ≤ 0.05) in untreated diabetic mice but in those given 80mg/kg ACAE were similar to saline controls. Diabetic controls showed degenerative changes in hepatocytes with disorganization of hepatic cords, congestion of central veins with mild hepatocellular necrosis, sinusoids infiltrated by mild nonspecific inflammatory cells. Hepatocytes revealed pyknosis, karyorrehexis, chromatolysis and cytoplasmic vacuolization. In mice given ACAE, we found few mildly degenerated hepatocytes around central veins with few cytoplasmic vacuoles but other hepatocytes and portal sinusoidal areas appeared normal. We found no hemorrhage, inflammatory cell infiltrations or parenchymal cell necrosis in livers of mice given ACAE. The findings suggest that 40-80mg/dl ACAE may reduce blood glucose levels and inhibit diabetes-induced liver damage in mice.
Keywords: Adiantum capillus-veneris, aqueous extract, hepatoprotective effects.