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OJVRTM

Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X

 

26 (10): 717-721, 2022.


Macro and microscopic effects of urotropine on egg embryos.

 

Hadi Tavakkoli 1, Amin Derakhshanfar 2, Sajedeh Salandari3

 

1,3Department of Avian Medicine, School of Veterinary Medicine, Shahid Bahonar University of Kerman, 2Center of Comparative & Experimental Medicine, School of Medicine, Shiraz University of Medical Sciences, Iran.

ABSTRACT

 

Tavakkoli H, Derakhshanfar A, Salandari S., Macro and microscopic effects of urotropine on egg embryos, Onl J Vet Res., (10): 717-721, 2022. Four groups of 10 fertile chicken eggs each were injected in yolk sac 10, 100, 200 and 300 mg/egg urotropine in 0.5ml phosphate buffer at 4th day incubation. Three groups of 10 controls were not injected, injected saline or buffer. Embryos were re-incubated post-treatment and viability monitored by candling. Dead embryos were excised for macro and microscopic lesions and live ones necropsied on day 18 of incubation. Control embryos appeared normal but those injected urotropine died before day 5 of incubation. By microscopy, we observed no lesions in controls, but in those given urotropine, found underdeveloped dilated renal tubules with hypertrophied glomeroli and prominent capillaries. We observed dose related gross and microscopic alterations in chicken embryos injected urotropine (P < 0.05) confined to renal structures.

 

Keywords: Chicken, Egg, Embryo, Toxicopathology, Urotropine.


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