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Online Journal of Veterinary Research©
Volume 23 (1): 9-14, 2019.
Frequency of sfa, cnf-1 and hly genes in Escherichia coli isolated in urine of patients with urinary tract infections.
Azadeh Foroughi1*, Shervin Ramezan-Ghanbari2
1Pathobiology & Basic Sciences Department, Veterinary Medicine Faculty, Razi University, Kermanshah,2Microbiology, Islamic Azad University, Broujerd Branch, Iran.
Foroughi A, Ramezan-Ghanbari S., Frequency of sfa, cnf-1 and hly genes in Escherichia coli isolated in urine of patients with urinary tract infections, Onl J Vet Res., 23 (1): 9-14, 2019. Uropathogenic E. coli (UPEC) can induce urinary tract infection (UTI) in humans. Cnf-1 gene encodes cytotoxic necrotizing factor, sfa, S. fimbriae and hly, hemolysin as urinary virulence factors. We confirmed E coli isolates by diagnostic and biochemical tests from 180 patients suspected with urinary tract infection. After extraction of DNA by phenol-chloroform, sfa, cnf-1 and hly genes were identified by PCR. We found 55.6% patients with UTI had E Coli, 74% were women aged 42.77 years and 26% men aged 45.88 years. The frequency of sfa was 27%, cnf-1 33% and hly genes 30%. Early detection of these virulence genes in urine can assist in treatment and prognosis of UTI, especially pyelonephritis.
Keywords: Uropathogenic Escherichia coli, hly, sfa, cnf-1.