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OJVRTM
Online Journal of Veterinary Research©
Volume 23 (1): 9-14, 2019.
Frequency of sfa, cnf-1 and hly
genes in Escherichia coli isolated in urine of patients with urinary
tract infections.
Azadeh Foroughi1*, Shervin Ramezan-Ghanbari2
1Pathobiology
& Basic Sciences Department, Veterinary Medicine Faculty, Razi University, Kermanshah,2Microbiology,
Islamic Azad University, Broujerd Branch, Iran.
ABSTRACT
Foroughi A, Ramezan-Ghanbari
S., Frequency of sfa, cnf-1 and hly
genes in Escherichia coli isolated in urine of patients with urinary tract
infections, Onl J Vet Res., 23 (1): 9-14, 2019. Uropathogenic E.
coli (UPEC) can induce urinary tract infection (UTI) in humans. Cnf-1 gene encodes cytotoxic necrotizing factor,
sfa, S. fimbriae and hly, hemolysin as
urinary virulence factors. We confirmed E
coli isolates by diagnostic and biochemical tests from 180 patients
suspected with urinary tract infection. After extraction of DNA by
phenol-chloroform, sfa, cnf-1 and hly genes were identified by PCR. We found 55.6%
patients with UTI had E Coli, 74% were women aged 42.77 years and 26% men aged
45.88 years. The frequency of sfa was 27%, cnf-1 33% and hly genes 30%. Early detection of these virulence genes
in urine can assist in treatment and prognosis of UTI, especially pyelonephritis.
Keywords: Uropathogenic
Escherichia coli,
hly, sfa,
cnf-1.
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