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OJVRTM

Online Journal of Veterinary Research

 

Volume 23 (7):717-723, 2019.


A silver nanoparticle antibacterial synthetized with Escherichia coli isolated from urine of dogs.

 

Dhuha Khalied Abd-Alameer and Aida Hussian Ibrahim.

 

ABSTRACT

 

Abd-Alameer DK, Ibrahim AH., A silver nanoparticle antibacterial synthetized with Escherichia coli isolated from urine of dogs, Onl J Vet Res., 23 (7):717-723, 2019. Urinary tract infections caused by antibiotic resistant bacteria are common in dogs. Nanoparticles can be used to replace antibiotics to inhibit bacteria. We used E coli isolated from urine of dogs to synthetize nanoparticles in a biomass culture in silver nitrate (AgNO3) solution. Nanoparticles were formed by reduction of aqueous silver nitrate confirmed by absorbance at 515 nm by UV-and presence of 10-100nm particles with electron microscopy. A broth dilution assay (BDA) for done to determine MIC of the silver nanoparticles against E coli. We detected MIC of ~66μg/ml with inhibition ~24mm.

 

Key-words: Nanoparticles, synthesis, E coli, urine,dogs, anti-bacterial.


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