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OJVRTM
Online Journal of
Veterinary Research©
Volume 23 (7):717-723, 2019.
A silver nanoparticle
antibacterial synthetized with Escherichia coli isolated from urine of dogs.
Dhuha Khalied Abd-Alameer and Aida Hussian
Ibrahim.
ABSTRACT
Abd-Alameer DK, Ibrahim
AH., A silver nanoparticle antibacterial synthetized with Escherichia coli isolated from urine of
dogs, Onl J Vet Res., 23 (7):717-723, 2019. Urinary
tract infections caused
by antibiotic resistant bacteria are common in dogs. Nanoparticles can be used
to replace antibiotics to inhibit bacteria. We used E coli isolated from urine of dogs to synthetize nanoparticles in a
biomass culture in silver
nitrate (AgNO3) solution. Nanoparticles were formed by
reduction of aqueous silver nitrate confirmed by absorbance at 515 nm by UV-and
presence of 10-100nm particles with electron microscopy. A broth dilution assay
(BDA) for done to determine MIC of the silver nanoparticles against E coli. We detected MIC of ~66μg/ml
with inhibition ~24mm.
Key-words:
Nanoparticles, synthesis, E coli, urine,dogs,
anti-bacterial.
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