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OJVRTM
Online Journal of Veterinary Research©
(Including Medical and
Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 29 (1): 14-24, 2025.
Single nucleotide polymorphism in 5'-untranslated lactoferrin gene
in cows with or without uterine infections.
A Hajibemani,
H Sharifiyazdi, A Mirzaei, A Rowshan Ghasrodashti.
1Department of Clinical Science, School of Veterinary Medicine, Shiraz
University, Shiraz 71345, Iran; 2Islamic Azad University, Kazerun
Branch, Kazerun, Iran
ABSTRACT
Hajibemani A, Sharifiyazdi
H, Mirzaei A, Ghasrodashti
AR., Single nucleotide polymorphism in
5'-untranslated lactoferrin gene in cows with or without uterine infections, Onl J Vet Res., 29
(1): 14-24, 2025. Lactoferrin
(Lf) antimicrobial protein is found in normal uterine discharges of cows and
allelic diversity may be associated with susceptibility or resistance to uterine infections.
We report single nucleotide polymorphism genes in 5' untranslated region
(5'-UTR,
position +32) of lactoferrin gene by allele-specific
PCR in cows with and without uterine infections. Blood was taken from 89
multiparous Holstein dairy cows with and 38 controls without history of uterine
infections. Results suggested presence of polymorphisms
(G > C) in 5'-UTR in all cows and allele specific PCR was 100% similar to
sequencing. We found no correlation between disease and SNP in 5'-UTR. However
we did find difference in mean reproductive parameters
of cows without polymorphism compared with SNP in 5'-UTR.
Cows with +32:CC genotype and +32:GC genotype SNP in
UTR had lower average of services per conception and days open compared with
+32:GG genotypes (P < 0.05). However, no significant difference in the
calving to first service was found between these genotypes (p > 0.05).
Keywords: Lactoferrin; polymorphism; uterine infection; cow