©1994-2018. All Rights Reserved. Online Journal of
Veterinary Research. You may not store these pages in any form except for your
own personal use. All other usage or distribution is illegal under
International copyright treaties. Permission to use any of these pages in any
other way besides the before mentioned must be gained in writing from the
publisher. This article is exclusively copyrighted in its entirety to OJVR
publications. This article may be copied once but may not be, reproduced or
re-transmitted without the express permission of the editor
OJVR
Online
Journal of Veterinary Research©
Volume 14 (1):56-65, 2010. Redacted
2017.
Molecular cloning and sequencing of Trypanosoma brucei rhodesiense
putative oligosaccharyl transferase
Waren N. Baticados1*(DVM, PhD), Noboru Inoue2 (DVM,
PhD), Chihiro Sugimoto3 (DVM, PhD), Hideyuki Nagasawa2
(DVM, PhD) and Abigail M. Baticados1 (DVM)
1College of Veterinary Medicine,University
of the Philippines Los Baños, Laguna; 2National
Research Center for Protozoan Diseases, Obihiro
University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido
8555, Japan and 3 Laboratory of Infectious Diseases, Graduate School
of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
ABSTRACT
Baticados
WN, Inoue N, Sugimoto C, Nagasawa H, Baticados AM Molecular Cloning and sequencing of
Trypanosoma brucei rhodesiense
Putative Oligosaccharyl transferase, Onl J Vet Res., 14 (1):56-65, 2010. Genomic clone of putative Trypanosoma brucei rhodesiense oligosaccharyl transferase (TbrOST I) with ~2000
bp DNA size was obtained by PCR amplification.
The size of the amplicon is akin to
other kinetoplastid homologues. Sequencing revealed a partial
nucleotide sequence of 2048 bp encoding a predicted
protein sequence of 682 amino acid residues. TbrOST I has a very high nucleotide (Ns) and amino acid (As) sequence identity
to putative oligosaccharyl transferase subunit
in the genomes of Trypanosoma brucei gambiense (Ns:98%; As:99%) and T. brucei
(Ns:94-98%; As:93-99%). However, only a moderately
high nucleotide identity and low amino acid sequence similarity to putative
oligosaccharyl transferase subunit was observed in
the genomes of Trypanosoma cruzi (Ns:74%; As:61%), Leishmania
infantum
(Ns:70-80%; As:46-54%), L. braziliensis (Ns:68-78%; As:46-55%) and L. major (Ns:69-76%; As:45-54%). Moreover, TbrOST I also exhibited moderately high sequence identity (71-77%) to the genome from other origins. Based
on the size of amplicon and high percent identity to reported or putative oligosaccharyl
transferase subunit in the genome of related species and a
variety of organisms, the results strongly indicate that TbrOST
I is a potential trypanosome oligosaccharyl transferase gene. The sequence data of TbrOST I has been deposited in the GenBank
with accession number GU475125.
Key words: Trypanosoma brucei
rhodesiense, putative oligosaccharyl
transferase, N-glycosylation
FULL-TEXT(SUBSCRIPTION OR PURCHASE ARTICLE)