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OJVRTM
Online
Journal of Veterinary Research ©
Volume 15 (1): 14-21, 2011. Redacted
2017.
Tissue
distribution of the enzyme rhodanese in rainbow trout
(Oncorhynchus mykiss), kutum (Rutilus frisii kutum)
and golden grey mullet (Liza aurata)
Baghshani
H1*, Shahsavani D 2
1Department of Basic Sciences and 2Department of Food Hygiene and Aquaculture , School of Veterinary
Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
ABSTRACT
Baghshani H, Shahsavani
D., Tissue distribution of the enzyme rhodanese in
rainbow trout (Oncorhynchus mykiss), kutum (Rutilus frisii kutum) and golden grey
mullet (Liza aurata), Onl J
Vet Res., 15
(1): 14-21, 2011. Rhodanese (thiosulfate: cyanide sulfurtransferase,
EC. 2.8.1.1) is a ubiquitous enzyme present in both prokaryotes and eukaryotes.
Its physiological function is controversial, but it is believed that the enzyme
plays a central role in cyanide detoxification. The purpose of this
investigation was to determine and compare the pattern of tissue distribution
of rhodanese in different tissues of three different
fish species including rainbow trout, kutum
and golden
grey mullet. In the three species studied, rhodanese
was present in all tissues albeit in different amounts. Specific activities
(enzyme units/mg protein) of rhodanese in different
tissues of rainbow trout, kutum and golden
grey mullet range 0.017–0.122, 0.049–0.219 and 0.062–0.258
respectively. The highest activity of rhodanese in
all three species was observed in liver and kidney. Among other tissues, rhodanese specific activity of intestine and gill was
considerably greater than the values of brain, heart and muscle in all studies
species. Significant inter-species differences were observed in the level of
enzyme in some tissues. Specific activity of rhodanese
was lowest in most tissues of rainbow trout in comparison with two other
species. The results of this study may
indicate the involvement of rhodanese in cyanide
detoxification in tissues that have greater potential to be exposed to higher
levels of cyanide. However, the widespread tissue distribution of rhodanese suggests additional important biological
functions for the enzyme besides cyanide detoxification.
Keywords: Rhodanese, Cyanide toxicity,
Detoxification, Fish species
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