days


2021-2033. All Rights Reserved. Online Journal of Veterinary Research . You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.


OJVRTM

Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X

 

Volume 27 (8): 477-483, 2023.


 

Effect of Trigonella seed extract on ischemic injury in male Wistar rats.

 

Omid Azari, Parham R Ebrahimi, Reza Kheirandish, Paidar Ardakani, Hamid Roshani.

 

School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

 

ABSTRACT

 

Azari O, Ebrahimi PR, Kheirandish R, Ardakani P, Roshani H., Effect of Trigonella seed extract on ischemic injury in male Wistar rats, Onl J Vet Res., 27 (8): 477-483, 2023. We report effect of Trigonella seed extract in groups of 6 male 280-300g Wistar male rats each with (1) or without (2) renal ischemia-reperfusion or gavaged 0.3g extract (3) daily for 14 days. Controls (1) underwent ischemia but were given saline only. Saline (controls) and Trigonella extract were gavaged daily 14 days before clamping aortic renal artery at day 14 for 45min, then unclamped for reperfusion for 1h. At day 15, left kidneys were removed and tissues subjected to microscopy. In rats given saline but clamped, we observed severe vacuolar degeneration, necrosis of proximal and distal tubules, cellular swelling, foamy cytoplasm, detachment of epithelial cells, nuclear pyknosis, glomerular atrophy, dilation, congestion and hemorrhage. Lesions were scored 0-5 and compared (P < 0.05) with unclamped saline controls, in clamped rats, tubular degeneration scored 3-4, tubular necrosis 2-3 and histopathology 3.5-5. However, in ischemic rats gavaged Trigonella we found only few proximal tubules exhibiting mild epithelial changes and tubular degeneration scored 1-2, tubular necrosis 0-2 and histopathology 0.5-2.5 (P < 0.05).

 

Key Words: Ischemia-reperfusion, kidney, Trigonella foenum graecum, Wistar rats.


MAIN

 

FULL-TEXT (SUBSCRIBE OR PURCHASE TITLE)