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OJVRTM
Online
Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 27
(8): 477-483, 2023.
Effect of Trigonella seed extract
on ischemic injury in male Wistar rats.
Omid
Azari, Parham R Ebrahimi, Reza Kheirandish, Paidar Ardakani, Hamid Roshani.
School of
Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
Azari O, Ebrahimi PR, Kheirandish R, Ardakani P, Roshani H., Effect of Trigonella seed extract on ischemic injury in
male Wistar rats, Onl J Vet
Res., 27 (8): 477-483, 2023. We report effect of Trigonella seed extract in groups
of 6 male 280-300g Wistar male rats each with (1) or
without (2) renal ischemia-reperfusion or gavaged
0.3g extract (3) daily for 14 days. Controls
(1) underwent ischemia but were given saline only. Saline (controls) and Trigonella
extract were gavaged daily 14 days before clamping
aortic renal artery at day 14 for 45min, then unclamped for reperfusion for 1h.
At day 15, left kidneys were removed and tissues subjected to microscopy. In rats given saline but clamped, we
observed severe vacuolar degeneration, necrosis of proximal and distal tubules,
cellular swelling, foamy cytoplasm, detachment of
epithelial cells, nuclear pyknosis, glomerular
atrophy, dilation, congestion and hemorrhage. Lesions were scored 0-5 and
compared (P < 0.05) with unclamped saline controls, in clamped rats, tubular
degeneration scored 3-4, tubular necrosis 2-3 and histopathology 3.5-5.
However, in ischemic rats gavaged Trigonella we found only few
proximal tubules exhibiting mild epithelial changes and tubular degeneration
scored 1-2, tubular necrosis 0-2 and histopathology 0.5-2.5 (P < 0.05).
Key Words: Ischemia-reperfusion, kidney, Trigonella
foenum graecum, Wistar rats.