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OJVRTM
Online Journal of Veterinary Research©
Volume
22 (3):237-246, 2018.
Effect of trehalose and
month of collection on DNA fragmentation in Holstein bull semen during
dilution, cooling and thawing.
¹*Kreem Iwaid
Al-Badry,²Qusay Mohammed Aboud,¹Sadeq Jaafer Zalzala, ³Faris Faisel Ibrahim, ³Wafa’a Yeedam
Lateef
¹Department of surgery
& Obstetrics-College of Veterinary Medicine- University of Baghdad; ²Ministry of Agriculture -Department
of Artificial Insemination center-Baghdad. *Part
of Master of Science thesis
ABSTRACT
Al-Badry
KI, Aboud QM, Zalzala SJ, Ibrahim FF, Lateef WY., Effect of trehalose
and month of collection on DNA fragmentation in Holstein bull semen during
dilution, cooling and thawing, Onl J Vet Res., 22 (3):237-246, 2018. We report optimal levels of trehalose in diluents for freezing bull semen monitored by
fragmentation of DNA during dilution, cooling and post thawing during October,
November, December and January. Ejaculates with 50-55% or more individual sperm
motility were collected weekly by artificial vagina. Semen was diluted with Tris containing 50, 100, 150 or 200 mM
Trehalose to provide 80 million spermatozoids per ml.
Semen was cooled to 5Cº over 4h, packed into 0.25 ml straws and freeze stored
48hr in liquid nitrogen. DNA fragmentation was evaluated after dilution,
cooling and thawing. After dilution, fragmentation in 50 (4.7%) or 100Mn (4.8%)
Trehalose was less (P < 0.05) compared with 200nM
(6.7%) or controls (6.7%). After cooling, fragmentation increased (P < 0.05) in all samples but
in those with 50nM was 6.6% less (P > 0.05) compared with 200nM (8.1%) or
controls 10.2%. After thawing fragmentation increased in all
samples but in those kept in 100nM Trehalose (7.4%)
was less (p>0.05). Our findings suggest that
50-100nm Trehalose in Tris-dilution
may reduce DNA fragmentation in sperm and ameliorate damage due to cooling or
freezing. We found that in semen collected in November and December, fragmentation
was less compared with other months.
Key words: Trehalose, dilution,
freezing, thawing, DNA fragmentation, bull semen.
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