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Online Journal of Veterinary Research


Volume 25(2): 124-130, 2021.

Effect of Mycobacterium bovis-tuberculosis BCG rabbit transfer factor on

methicillin resistant staphylococci (MRSA) in rats.


Hussein Emad Abdulrazaq1 MSc, Mawlood Abbas Ali Al-Graibawi 2 PhD.


Zoonosis diseases Unit 1, Department of Internal and Preventive medicine2, College of Veterinary Medicine, University of Baghdad, Iraq




Abdulrazaq HE, Ali Al-Graibawi MA., Effect of Mycobacterium bovis-tuberculosis BCG transfer factor vaccine on antibiotic resistant staphylococci in rats, Onl J Vet Res., 25(2): 124-130, 2021. We describe effect of Mycobacterium bovis BCG transfer factor (TF) generated in rabbit splenocytes on experimental Methicillin Resistant Staphylococcus Aureus (MRSA) infection in rats. To generate TF, groups of 6 rabbits were vaccinated subcutaneous (SC) at 7 and 14 days BCG tuberculosis vaccine. Controls were vaccinated only phosphate buffer saline (PBS). Rabbits were examined daily for clinical signs and delayed hypersensitivity test (DTH). Those given BCG had positive skin test reactions. Then, TF from rabbit spleen leucocytes were tested for immune response in non-sensitized rats. Three groups of 10 albino rats each were injected SC 0.5ml TF 5x108 cells/ml, 0.5ml BCG (positive control) or 0.5ml PBS (negative control) and later tested for DTH. Those injected TF or BCG, reacted with DTH whereas controls did not. One week later, rats were challenged intraperitoneally 0.5mL of 5xCFU MRSA. Survival in those given TF was 100%, BCG 70% and controls 0%. Our results suggest that rabbit TF could transfer DTH to recipient non-sensitized rats and enhance immune reaction to MRSA in rats.


Keywords | BCG, Transfer factor, Immunotherapy, DTH- skin test, Cellular immunity.