2021-2032 All Rights Reserved. Online Journal of Veterinary Research.  You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to onlinejournals@gmail.com publications. This article may be copied once but may not be, reproduced or  re-transmitted without the express permission of the editors. Linking: To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.


OJVRTM

Online Journal of Veterinary Research

 

Volume 25(2): 124-130, 2021.


Effect of Mycobacterium bovis-tuberculosis BCG rabbit transfer factor on

methicillin resistant staphylococci (MRSA) in rats.

 

Hussein Emad Abdulrazaq1 MSc, Mawlood Abbas Ali Al-Graibawi 2 PhD.

 

Zoonosis diseases Unit 1, Department of Internal and Preventive medicine2, College of Veterinary Medicine, University of Baghdad, Iraq

 

ABSTRACT

 

Abdulrazaq HE, Ali Al-Graibawi MA., Effect of Mycobacterium bovis-tuberculosis BCG transfer factor vaccine on antibiotic resistant staphylococci in rats, Onl J Vet Res., 25(2): 124-130, 2021. We describe effect of Mycobacterium bovis BCG transfer factor (TF) generated in rabbit splenocytes on experimental Methicillin Resistant Staphylococcus Aureus (MRSA) infection in rats. To generate TF, groups of 6 rabbits were vaccinated subcutaneous (SC) at 7 and 14 days BCG tuberculosis vaccine. Controls were vaccinated only phosphate buffer saline (PBS). Rabbits were examined daily for clinical signs and delayed hypersensitivity test (DTH). Those given BCG had positive skin test reactions. Then, TF from rabbit spleen leucocytes were tested for immune response in non-sensitized rats. Three groups of 10 albino rats each were injected SC 0.5ml TF 5x108 cells/ml, 0.5ml BCG (positive control) or 0.5ml PBS (negative control) and later tested for DTH. Those injected TF or BCG, reacted with DTH whereas controls did not. One week later, rats were challenged intraperitoneally 0.5mL of 5xCFU MRSA. Survival in those given TF was 100%, BCG 70% and controls 0%. Our results suggest that rabbit TF could transfer DTH to recipient non-sensitized rats and enhance immune reaction to MRSA in rats.

 

Keywords | BCG, Transfer factor, Immunotherapy, DTH- skin test, Cellular immunity.


MAIN

 

FULL-TEXT (SUBSCRIBE OR PURCHASE TITLE)