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OJBTM
Online
Journal of Bioinformatics
©
Volume 14 (1): 66-75,
2013
Identification of single nucleotide polymophisms
using CLC and SeqMan transcriptome sequencing data.
Manisha
R.
Sajnani, Vaibhav
D. Bhatt and
Chaitanya G. Joshi*
Department of Animal
Biotechnology, College of
Veterinary Science and Animal Husbandry, Anand
Agricultural
University, Gujarat,
India.
ABSTRACT
Sajnani MR,
Bhatt VD, Joshi CG., Identification of single nucleotide polymophisms
using CLC and SeqMan transcriptome
sequencing data, Online J Bioinform.,
14 (1): 66-75,
2013. Next generation
sequencing (NGS)
technologies produce very large amount of data at low cost.
Single Nucleotide
Polymorphisms (SNPs) are the most abundant form
of genetic
variation and need to
be identified
quickly. The performance of CLC and SeqMan for prediction of potential SNPs
from human buccal cancer and
healthy transcriptome
data obtained from Roche 454 sequencing technology based on
software utility,
time, memory, disk space and accuracy are described.
Keywords: Sequencing,
Single
Nucleotide Polymorphisms, CLC, SeqMan,
Buccal, Transcriptome