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Online Journal of Veterinary Research©

Volume 21(9):635-642, 2017.

 Effect of Tranilast on morphine-induced MCF-7 cell viability and death.


Vahid Farnia1, Hossein Zhaleh1*, Mohammad Mehdi Zangeneh2, Akram Zangeneh3, Zhaleh Shahmoradi1


1Substance Abuse Prevention Research Center, Kermanshah University of Medical Sciences, Kermanshah, Department(s) of 2Clinical Sciences, 3Microbiology, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran. *Corresponding Author:




Farnia V, Zhaleh H, Zangeneh MM, Zangeneh A, Shahmoradi Z., Effect of Tranilast on morphine-induced MCF-7 cell viability and death. Onl J Vet Res.,  21(9):635-642, 2017.  The anti-allergic drug Tranilast may induce cell line proliferation and prevent apoptosis. MCF-7 cells were grown in Dulbecco's modified eagle's medium, fetal bovine serum, non-essential amino acid, L-glutamine, penicillin and streptomycin in T-25 cm2 tissue culture flasks. Cells were plated in 24 well culture plates with 104 cells/mL densities for 12h, further cultured in Tranilast media for 24h. Percentage viability was measured at 490-492nm by ELISA. Morphine was used as an apoptosis control.  One day after plating, cells were washed with PBS. Effect of Tranilast on viability and cell death was determined by microscopy, trypan blue, Hoescht/PI staining and lactate dehydrogenase test. Data analyzed using one-way ANOVA followed by Tukey’s post hoc tests suggested that Tranilast increased cell viability and decreased cell death (p<0.05).


Keywords: Tranilast, morphine, cell viability, cell death.