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Online Journal of Veterinary Research©
Volume 23 (4):285-298, 2019.
Toxoplasma gondii and OP5 and GRA6 virulence factors isolated from ovine blood and muscle exudate.
Sahad MK Al-Dabbagh, Haider MA Al-Rubaie.
Department of Parasitology, College of Veterinary Medicine, University of Baghdad -Iraq
Al-Dabbagh SMK, Al-Rubaie HMA., Toxoplasma gondii and OP5 and GRA6 virulence factors isolated from ovine blood and muscle exudate, Onl J Vet Res., 23 (4):285-298, 2019. We determined incidence of T Gondii in 48 blood and raw meat sheep samples by RT-PCR. B1 gene in T gondii ROP5 and GRA6 virulence factors by location, age, gender and season are also reported. We found T gondii in ~19% blood samples with no difference between ROP5 and GRA6 expression. Age, gender, location or season had no effect on incidence for T Gondii but found we found significant (P < 0.05) differences with ROP5 (over-expression) and GRA6 gene (down regulation). In meat, we found 33% samples infected by T Gondii, but no difference in ROP5 and GRA6.
Key word: Toxoplasma gondii; gene expression; virulence factors ROP5 and GRA6; sheep.