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OJVRTM
Online Journal of Veterinary Research©
Established 1994
ISSN 1328-925X
Volume 22 (8):703-706, 2018.
Prevalence of Toxoplasma gondii in free range eggs detected by smear
impression and PCR.
May Najii Al-Khanaq, Dunya Talib Al-Rawdan, and Zina Shakir Shallal.
Department of
Biology, College of Science, University of Wasit,
Iraq.
,
ABSTRACT
Al-Khanaq MN, Al-Rawdan
DT, Shallal ZS., Prevalence of Toxoplasma gondii in free range eggs detected by smear impression and
PCR, Onl J Vet Res., 22 (8):703-706, 2018.
We report prevalence of Toxoplasma gondii in 30 free-range
chicken eggs detected by impression smear and PCR. By smearing we found 17
positive eggs but by PCR only 6. Upon the direct microscope examination of yolk
eggs smear cysts were spherical shaped Bradyzoites.
We surmise that high incidence in our study was due to presence of cats which
defecate oocysts in proximity to chickens. PCR may not confirm T. gondii due to loss of some or all the
gene during extraction, and genomic DNA of host and parasite may be
insufficient to amplify on agarose gel. This may account for our lower
incidence by PCR. In this case we must assume that the smear method was
probably more reliable. However results suggest viable T. gondii in raw free range eggs.
Keywords:
Toxoplasmosis, PCR, chickens, impression smears.
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