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Online Journal of Veterinary Research©
Volume 22 (8):703-706, 2018.
Prevalence of Toxoplasma gondii in free range eggs detected by smear impression and PCR.
May Najii Al-Khanaq, Dunya Talib Al-Rawdan, and Zina Shakir Shallal.
Department of Biology, College of Science, University of Wasit, Iraq.
Al-Khanaq MN, Al-Rawdan DT, Shallal ZS., Prevalence of Toxoplasma gondii in free range eggs detected by smear impression and PCR, Onl J Vet Res., 22 (8):703-706, 2018. We report prevalence of Toxoplasma gondii in 30 free-range chicken eggs detected by impression smear and PCR. By smearing we found 17 positive eggs but by PCR only 6. Upon the direct microscope examination of yolk eggs smear cysts were spherical shaped Bradyzoites. We surmise that high incidence in our study was due to presence of cats which defecate oocysts in proximity to chickens. PCR may not confirm T. gondii due to loss of some or all the gene during extraction, and genomic DNA of host and parasite may be insufficient to amplify on agarose gel. This may account for our lower incidence by PCR. In this case we must assume that the smear method was probably more reliable. However results suggest viable T. gondii in raw free range eggs.
Keywords: Toxoplasmosis, PCR, chickens, impression smears.