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OJVRTM
Online Journal of Veterinary Research©
(Including Medical and
Laboratory Research)
Established
1994
ISSN 1328-925X
Volume 27 (1): 37-45,
2023.
PCR for mec-A gene for equine toxic shock syndrome
and effect of mare, cow and goat milk or colostrum
probiotics on drug resistant staphylococci.
Sherein I Abd El-Moez1, Ahmed FY2,
Wagdy KB, Khalil3
1Department of Microbiology and Immunology, 2Department
of Animal Reproduction and A.I, 3Department of Cell Biology,
National Research Center, Giza, Egypt.
ABSTRACT
El-Moez
SA, Ahmed FY, Khalil WK., PCR
for mec-A gene for equine toxic shock syndrome and
effect of mare, cow and goat milk or colostrum probiotics on drug resistant
staphylococci, Onl J Vet Res., 27
(1): 37-45, 2023. Authors found 19% mortality in a herd of mixed breed horses with
multiple drug resistant S. aureus (MRSA) in excretions, tissues and carcasses. Mec-A gene was detected by PCR to confirm MRSA. For Probiotics from
colostrum and milk against MRSA were tested by agar well diffusion test. Results
revealed that L. acidophilus isolated from colostrums of mare showed most
inhibition of MRSA, followed by L.acidophilus isolated from goat colostrum, Bifidobacterium and L.
acidophilus from Buffalo-cow milk. We
find mec-A gene from MRSA strains to be primary cause of
fatal toxic shock syndrome outbreak in horses subject to stress with symptoms
of generalized toxaemia.
Keywords:
MRSA; Quantitative-PCR; mec-A gene; Toxic Shock Syndrome; Equine; Probiotic.