2021-2033. All Rights Reserved. Online Journal of Veterinary Research . You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.


OJVRTM

Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X

 

Volume 27 (1): 37-45, 2023.


PCR for mec-A gene for equine toxic shock syndrome and effect of mare, cow and goat milk or colostrum

probiotics on drug resistant staphylococci.

Sherein I Abd El-Moez1, Ahmed FY2, Wagdy KB, Khalil3

 

1Department of Microbiology and Immunology, 2Department of Animal Reproduction and A.I, 3Department of Cell Biology, National Research Center, Giza, Egypt.

 

ABSTRACT

El-Moez SA, Ahmed FY, Khalil WK., PCR for mec-A gene for equine toxic shock syndrome and effect of mare, cow and goat milk or colostrum probiotics on drug resistant staphylococci, Onl J Vet Res., 27 (1): 37-45, 2023. Authors found 19% mortality in a herd of mixed breed horses with multiple drug resistant S. aureus (MRSA) in excretions, tissues and carcasses. Mec-A gene was detected by PCR to confirm MRSA. For Probiotics from colostrum and milk against MRSA were tested by agar well diffusion test. Results revealed that L. acidophilus isolated from colostrums of mare showed most inhibition of MRSA, followed by L.acidophilus isolated from goat colostrum, Bifidobacterium and L. acidophilus from Buffalo-cow milk. We find mec-A gene from MRSA strains to be primary cause of fatal toxic shock syndrome outbreak in horses subject to stress with symptoms of generalized toxaemia.

 

Keywords: MRSA; Quantitative-PCR; mec-A gene; Toxic Shock Syndrome; Equine; Probiotic.