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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 27 (1): 37-45, 2023.
PCR for mec-A gene for equine toxic shock syndrome and effect of mare, cow and goat milk or colostrum
probiotics on drug resistant staphylococci.
Sherein I Abd El-Moez1, Ahmed FY2, Wagdy KB, Khalil3
1Department of Microbiology and Immunology, 2Department of Animal Reproduction and A.I, 3Department of Cell Biology, National Research Center, Giza, Egypt.
El-Moez SA, Ahmed FY, Khalil WK., PCR for mec-A gene for equine toxic shock syndrome and effect of mare, cow and goat milk or colostrum probiotics on drug resistant staphylococci, Onl J Vet Res., 27 (1): 37-45, 2023. Authors found 19% mortality in a herd of mixed breed horses with multiple drug resistant S. aureus (MRSA) in excretions, tissues and carcasses. Mec-A gene was detected by PCR to confirm MRSA. For Probiotics from colostrum and milk against MRSA were tested by agar well diffusion test. Results revealed that L. acidophilus isolated from colostrums of mare showed most inhibition of MRSA, followed by L.acidophilus isolated from goat colostrum, Bifidobacterium and L. acidophilus from Buffalo-cow milk. We find mec-A gene from MRSA strains to be primary cause of fatal toxic shock syndrome outbreak in horses subject to stress with symptoms of generalized toxaemia.
Keywords: MRSA; Quantitative-PCR; mec-A gene; Toxic Shock Syndrome; Equine; Probiotic.