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Online Journal of Veterinary Research©
Volume 22 (6):486-494, 2018
Prevalence and identity of Toxocara canis isolates in stray dogs (Iraq).
Noor Idan Jarad, Athmar Kdair Abbas.
Department of Parasitology /College of Veterinary Medicine/University of Baghdad/Iraq.
Jarad NI, Abbas AK., Prevalence and identity of Toxocara canis isolates in stray dogs (Iraq). Onl J Vet Res., 22 (6):486-494, 2018. We determined prevalence of Toxocara canis in Southern Iraq during January to September 2017 in 270 stray dogs by molecular and microscopic methods. Effect of gender, age and season on prevalence was recorded. By fecal flotation method we found ~23% to be infected of which 23.7% were males and 22.8% female with no statistical difference. Prevalence peaked at 40% during February with temperature of ~26º C and humidity of ~30%. Incidence was higher (P < 0.05) in younger dogs (34.9%) compared with adults (15.8%). By PCR, prevalence was 58% with 10 T. canis identical isolates (accession no. KF577855.1) as found in Iran, while identity of 99% for T.canis3 and 4. Results revealed that the Internal Transcribed Spacer (ITS1) regions sequence of the 10 isolates showed 99% homology with reference sequences of T. canis from China (accession no. JF837169.1).
Keywords: Prevalence, Toxocara canis , Isolates, risk factors, PCR, Phylogeny.