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Online Journal of Veterinary Research

Established 1994 
ISSN 1328-925X


Volume 22 (9):772-783, 2018.

Pathology and toll Like Receptor-4 in acute and chronic fasciolasis in sheep.


1Manaf Ali Jasim, 2Huda Sadoon Al-Biaty, 3 Thaer Jawad Khadim

1Department(s) of Parasitology, 2Microbiology, College(s) of Veterinary Medicine, University of Baghdad, 3Yarmouk Hospital, Ministry of Health , Iraq




Jasim MA, Al-Biaty HS, Khadim TJ., Pathology and toll Like Receptor-4 in acute and chronic fasciolasis in sheep, Onl J Vet Res., 22 (9):772-783, 2018. Authors describe pathology and expression of TLR-4 in liver bile duct tissues and fibroblasts of 120 sheep with or without fasciola hepatica. Migration of young flukes severely damaged parenchyma with perforations of capsule in the acute phase. In the chronic phase, livers were nodular fibrotic with hypertrophied obstructed (fluke) bile ducts and calcification. We detected TLR-4 in liver tissue (n = 12) infested by Fasciola (P < 0.004) but none in normal sheep (n = 108). Nine of the 12 cases exhibited over expression of TLR-4, with none in normal sheep (P < 0.0004). TLR-4 was detected in all acute cases but only in ~63% (P < 0.01) of chronic ones. TLR-4 was located in the cytoplasm of hepatocytes, fibroblasts and biliary epithelium cells. Our results suggest that TL4-R may be a useful marker of acute and chronic fasciolasis.

Key words:Toll like receptor, Fasciola hepatica, Immunohistochemistry.