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OJVRTM

Online Journal of Veterinary Research©

 

Volume 19(1): 38-46, 2015. Redacted 2018.


Cloning, transformation and expression of infectious bursal disease virus VPX gene in Nicotiana tabacum.

 

Karami-Hoomani M1, Rajabi-Memari H1,2, Nabati-Ahmadi D1, Hosseni S.D3, Kheirandish M4

 

1,4School of Agriculture,  2Biotechnology and Life Science Center, Shahid Chamran University, Ahvaz, 3Razi Vaccine and Serum Research Institute, Arak, Iran

 

ABSTRACT

 

Karami-Hoomani M, Rajabi-Memari H, Nabati-Ahmadi, Hosseni SD, Kheirandish M., Cloning, transformation and expression of infectious bursal disease virus VPX gene in Nicotiana tabacum, Onl J Vet Res., 19(1): 38-46, 2015., Infectious bursal disease virus (IBDV) is a highly immunosuppressive contagious disease in young chickens. Precursor VP2 protein (VPX), a primary host protective immunogene of IBDV  could be used as subunit DNA vaccine against IBDV. VPX gene of IBDV was amplified by PCR and cloned into plant expression vector pCAMBIA1305.1. Agrobacterium tumefaciens with the recombinant construct was then transformed in leaf disk of Nicotiana tabacum cv. Xanthi  plant. Transgenic lines were screened on media containing cephotaxime (500 mgl-1) and hygromycin (15 mgl-1). Expressed VPX protein in the transgenic plants was confirmed by dot blotting and Western blot analyses. The findings suggest that a stable expression of VPX protein in tobacco plant is possible.

 

Key words: Infectious bursal disease virus (IBDV), expression, VPX, Agrobacterium tumefaciens , Nicotiana tabacum.


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