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OJVRTM
Online Journal of Veterinary Research©
Volume 19(1):
38-46, 2015. Redacted 2018.
Cloning, transformation and expression
of infectious
bursal disease virus VPX gene in Nicotiana tabacum.
Karami-Hoomani M1, Rajabi-Memari H1,2, Nabati-Ahmadi
D1, Hosseni
S.D3, Kheirandish M4
1,4School
of Agriculture, 2Biotechnology
and Life Science Center, Shahid Chamran
University, Ahvaz, 3Razi Vaccine and Serum Research Institute, Arak,
Iran
ABSTRACT
Karami-Hoomani M, Rajabi-Memari H, Nabati-Ahmadi, Hosseni SD,
Kheirandish M., Cloning, transformation and expression of
infectious bursal disease virus VPX gene in Nicotiana
tabacum, Onl J Vet Res., 19(1):
38-46, 2015., Infectious bursal disease virus (IBDV) is a highly
immunosuppressive contagious disease in young chickens. Precursor VP2 protein
(VPX), a primary host protective immunogene of IBDV could be used as
subunit DNA vaccine against IBDV. VPX gene of IBDV was amplified by PCR and
cloned into plant expression vector pCAMBIA1305.1. Agrobacterium tumefaciens with the recombinant
construct was then transformed in leaf disk of Nicotiana
tabacum cv. Xanthi plant.
Transgenic lines were screened on media containing cephotaxime
(500 mgl-1) and hygromycin
(15 mgl-1). Expressed VPX protein in the transgenic plants was confirmed by dot
blotting and Western blot analyses. The findings suggest that a stable
expression of VPX protein in tobacco plant is possible.
Key words: Infectious bursal disease virus (IBDV), expression,
VPX, Agrobacterium tumefaciens , Nicotiana tabacum.
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