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OJVRTM
Online Journal of Veterinary Research©
Volume 13 (1):86-93, 2009. Editor
note: Extensively Redacted 2018.
Polymorphism in TNP-2 gene and its association with
seminal parameters of cattle and buffalo bulls.
Panigrahi SK, Yadav BR
Livestock Genome Analysis Laboratory, Dairy Cattle Breeding
Division, National Dairy Research Institute, Karnal -
132 001, Haryana, India
ABSTRACT
Panigrahi SK, Yadav BR,
Polymorphism
in TNP-2 gene and its association with seminal parameters of cattle and buffalo
bulls, Onl J Vet Res, 13 (1):86-93, 2009. In the process
of sperm maturation, major restructuring of somatic chromatin wherein histones are replaced by a
group of arginine and lysine-rich transition proteins (TPs); which are in turn
replaced by protamines, occurs. Transition nuclear protein-2 (TNP-2) gene was analyzed by PCR-SSCP to
detect polymorphism in Sahiwal, Murrah and Karan
Fries bulls. Sequence analysis
of TNP-2 gene of Murrah buffalo bulls
revealed two single nucleotide polymorphisms (SNPs) at 182 bp and 186 bp position of intronic region. However, the gene sequences of Sahiwal and
Karan-Fries bulls revealed no variation.
The effect of this polymorphism was explored on individual motility, mass
activity and maturation of spermatozoa. Analysis of variance indicates
two variants of Murrah buffalo have no significant
effect on maturation, mass activity and individual motility of spermatozoa.
However, breed had a significant effect on mass activity and individual
motility, but not on sperm maturation.
Key words: Polymorphism, SSCP, TNP-2 gene, sperm maturation, cattle,
buffalo, bulls.
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