1994-2018 All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under International copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editor


Online Journal of Veterinary Research

Volume 22 (1):7-14, 2018.

Phylogenetic analysis of Toll Like Receptor 4 gene in cattle.


1Zahra Biranvad, 2*Ziaeddin Mirhoseini, 3*Mostafa Ghaderi-Zefrehei, 4Arash Fazeil, 2Seyed Hossein Hosseini Moghadam, 5Kianoush Zarrin Kavyani


1PhD student, Guilan University, Rasht, 2Professor in molecular genetics, Guilan University, Rasht, 3Assistance Professor in systems biology, Yasouj University, Yasouj, 4Assistance Professor in molecular genetics, Ilam University, Ilam, 5Faculty member Ilam University, Ilam, Iran. *Corresponding author: Ziaaldin Mir Hosseini, Email:




Biranvad Z, Hosseini ZM, Ghaderi-Zefrehei M, Fazeil A, Hosseini Moghadam SH, Zarrin Kavyani K., Phylogenetic analysis of Toll Like Receptor 4 genes in cattle. Onl J Vet Res., 22 (1):7-14, 2018. Detection of candidate genes involved in immune response in cattle may assist in recognizing species specific response to pathogens. Toll-like receptors (TLRs) have a vital role in disease resistance through their recognition of pathogen associated molecular patterns. We describe nucleotide variation and phylogenetic analysis of TLR4 gene in 29 species extracted from NCBI. Sequences were aligned by Clastalw and a similarity, distance and phylogentic tree was constructed with Megalign. cDNA sequence alignment for TLR4 revealed sequences of high similarity and one small region, possibly a mutation. Results suggested high variation of nucleotides among sequences. Ceratothorium simum simum showed 99% similarity and Felis silvestris catus 97.3% similarity, but Mus musculus only 21.4% with Homo sapiens. Cattle with allele A in T4CRBR1 showed lower somatic cell score than allele B (P < 0.05). Higher variability was identified in the extracellular region of the TLR4 protein, accounting for 88.79% (AA). Findings showed that TLR4 in Equus caballus was very close to Bos Taurus and could possibly be used for selecting mastitis breeding programs for cows.


KEYWORDS: TLR4, Phylogenetic analysis, cattle.