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OJVRTM
Online Journal of Veterinary Research ©
Volume 14 (2): 218-226, 2010. Redacted 2017.
Identification and sequence analysis of TLR2 gene in Murrah Buffalo.
Animal Genomics Lab, Department of Animal Biotechnology, GADVASU,
Ludhiana, Punjab-141004
ABSTRACT
Shah
SM, Kumar R, Brah GS
Identification and sequence analysis of TLR2 gene in Murrah
Buffalo, Onl J Vet Res 14 (2): 218-226, 2010.
Toll-like receptor 2 (TLR2) the most promiscuous of all the TLRs and has been
implicated in signaling induced by gram-positive cell walls, peptidoglycan and
mycobacterial factors, GPI anchors (Trypanosoma
cruzi), lipoarabinomann
(Mycobacterium tuberculosis), porins (Nesseria meningitides)
and zymosan (yeast cell wall component) and a number
of endogenous ligands viz., necrotic cells and their protein byproducts and
heat shock proteins (HSP60, HSP 70 and GP96). The present study has
characterized TLR2 gene in Murrah buffalo (Bubalus bubalis). A 1152bp partial coding sequence of TLR2 gene was amplified,
cloned, sequenced and characterized. The sequence was submitted to Genbank with accession number GU441859. The sequence shared
98% identity with that of Capra hircus, 97% with Bos taurus, Bos indicus and Bison bison,
96% with Boselaphus tragocamelus,
94% with Ovis aries, 82%
with Sus scrofa and Eqqus cabalus, and
81% with Canis lupus familaris.
The deduced protein sequence showed 95% identity with Bison bison, 94% identity with Bos indicus and Bos taurus,
93% with Boselaphus
tragocamelus, 90% with Ovis aries, 89 % with Capra hircus, 73% with Sus scrofa, 71%
with Equus caballus and Canis lupus familaris
and 44% with Gallus gallus.
Phylogenetic analysis of the deduced amino acid sequences of various species, revealed that buffalo clustered together with other
bovids and Capra
hircus, and away from Equus cabalus, Sus scrofa and Gallus gallus depicting the genetic
relatedness and divergence among various species.
Analysis of the number of synonymous substitutions per synonymous site
and non synonymous substitutions per non synonymous
site indicated that the nucleotide sequences coding for the TLR2 gene were not
under positive selection and null hypothesis of strict-neutrality was accepted.
Key words: Cloning, Characterization,
Toll like receptor, Buffalo, Phylogeny.