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OJVRTM
Online Journal of
Veterinary Research©
Volume 16 (3):117-122, 2012. Redacted
2017.
Molecular surveillance by PCR 16S and 18S rRNA
genes for bovine tick-borne diseases
Naghshgar N1, Namavari
M2.
1 Islamic Azad University of Jahrom,
2Razi Vaccine and Serum Research Institute, Shiraz, Iran
ABSTRACT
Naghshgar
N, Namavari M., Molecular surveillance by PCR 16S and
18S rRNA genes for bovine tick-borne diseases, Online
J Vet Res., 16 (3):117-122, 2012.
Tick-borne diseases in cattle are common in temperate, subtropical and
tropical countries and lead to meat and milk production losses with some
diseases being zoonozes. However, little is known
about the pathogens and no molecular method allows an integrated detection of
tick-borne pathogens in cattle in Iran. In this study we monitored 100 cattle
from different regions of Fars province Iran. The molecular surveillance of
tick-borne diseases was based on PCR amplification of the 16S and 18S rRNA genes. For rickettsia, PCR was positive for 2% of
blood samples, whereas for protozoa, PCR was positive for 19% of blood samples.
Two of the bacteria-positive blood samples were also positive for protozoa.
Sequencing results showed that cattle were infected with Anaplasma marginale and Theileria annulata .
Keywords: Tick
borne disease , Cattle, Molecular diagnosis
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