Plasma superoxide dismutase, malondialdehyde and lymphocyte DNA damage in horses with Theileria equi detected by qPCR.

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OJVRTM

Online Journal of Veterinary Research©

Volume 22 (6):457-466, 2018.

Plasma superoxide dismutase, malondialdehyde and lymphocyte DNA damage

in horses with Theileria equi detected by qPCR.

Hayder Dawood Saleem, Firas Rashad Al-Samarai.

Department of Veterinary Public Health, College of Veterinary Medicine, University of Baghdad, Iraq

ABSTRACT

Saleem HD, Al-Samarai FR., Plasma superoxide dismutase, malondialdehyde and lymphocyte DNA damage in horses with Theileria equi detected by qPCR, Onl J Vet Res., 22 (6):457-466, 2018. Plasma superoxide dismutase (SOD) and malondialdehyde (MDA) levels and DNA damage in lymphocytes of horses with Theileria equi detected by qPCR is reported. Ninety four blood samples were collected from different locations in horses in Iraq and subjected to real-time PCR (qPCR) for detection of a 450bp long section of 18S rRNA. Thirty horses were infected accounting for a prevalence of 31.91%. Then, plasma SOD and MDA concentrations and comet assay for DNA damage in mononuclear cells were determined in 30 infected and 30 non-infected controls to construct a Receiver Operation characteristic curve (ROC curve). The ROC curve showed sensitivity of 93% and specificity of 100% for MDA and 66.6% and 100% for SOD. Controls had no DNA damage in 50.39% lymphocytes, low in 39.69%, moderate in 6.25% and high damage in 3.38% whereas in those infected by T. equi we found no damage in 34.14%, low in 28.63, moderate in 21.11% and high damage in 16.09% in cells of horses. The findings suggest that MDA and SOD may be a reliable detector for Theileria equi in horses and comet assay for DNA damage in equine lymphocytes.

Keywords: Equine piroplasmosis, SOD, MDA, comet assay.