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Online Journal of Veterinary Research©
Volume 23 (5):383-394, 2019.
Incidence of Echinococcus granulosus in stray dogs by microscopy and PCR.
Haidear Barakat Abbass Al-Azzawi, Athmar Khdair Abbass Al-Azzawi.
Department of parasitology, College of Veterinary Medicine, University of Baghdad, Iraq.
Abbass Al-Azzawi HB, Khdair Abbass Al-Azzawi A., Incidence of Echinococcus granulosus in stray dogs by microscopy and PCR, 23 (5):383-394, 2019. We determined prevalence of Echinococcus granulosus in stray dogs (n=200) in Baghdad, Iraq, by microscopic methods and PCR (n=101). A primer using a complete sequence Echinococcus granulosus ND1 protein subunit of mitochondrial NADH 3,307-4,262bp was used for PCR. By direct wet smear we found ~24%, flotation ~34% but by PCR ~90% infection (P < 0.05). By microscopy, incidence was ~25% in males, ~42% females, adults ~43% and puppies ~22% (P < 0.05). This high prevalence in stray dogs in Baghdad city may be attributed to contamination by poor hygiene, behavior, lack of treatment, carcass and expedited animal offal. PCR will detect any DNA of the parasite which may account for the high incidence value for Echinococcus granulosus with specific primers derived from ND1 gene.
Keywords: Prevalence, stray dogs Echinococcus granulosus, microscopy, PCR.