1994-2020. All Rights Reserved. Online Journal of Veterinary Research. You may not store
these pages in any form except for your own personal use. All other usage or
distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the
before mentioned must be gained in writing from the publisher. This
article is exclusively copyrighted in its entirety to OJVR publications. This
article may be copied once but may not be, reproduced or re-transmitted without
the express permission of the editors.
OJVRTM
Online
Journal of Veterinary Research©
Volume 23 (5):383-394, 2019.
Incidence of
Echinococcus granulosus
in stray dogs by microscopy and PCR.
Haidear
Barakat Abbass Al-Azzawi, Athmar Khdair Abbass Al-Azzawi.
Department of parasitology, College of
Veterinary Medicine, University of Baghdad, Iraq.
ABSTRACT
Abbass Al-Azzawi
HB, Khdair Abbass Al-Azzawi
A., Incidence of Echinococcus granulosus
in stray dogs by microscopy and PCR, 23
(5):383-394, 2019. We determined prevalence of Echinococcus granulosus
in stray dogs (n=200) in Baghdad,
Iraq, by microscopic methods and PCR (n=101). A primer using a complete
sequence Echinococcus granulosus
ND1 protein subunit of mitochondrial NADH 3,307-4,262bp was used for PCR. By
direct wet smear we found ~24%, flotation ~34% but by PCR ~90% infection (P
< 0.05). By microscopy, incidence was ~25% in males, ~42% females, adults
~43% and puppies ~22% (P < 0.05). This
high prevalence in stray dogs in Baghdad city may be attributed to contamination
by poor hygiene, behavior, lack of treatment, carcass and expedited animal
offal. PCR will detect any DNA of the parasite which may account for the high
incidence value for Echinococcus granulosus with specific primers derived from ND1 gene.
Keywords: Prevalence, stray dogs Echinococcus granulosus,
microscopy, PCR.
FULL-TEXT (SUBSCRIPTION OR
PURCHASE TITLE)