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OJVRTM
Online Journal of
Veterinary Research©
Volume
24
(3): 150-158, 2020.
Mesenchymal stem cell treatment for superficial digital flexor tendon
injury in equine athletes.
Patrícia Furtado Malard¹ (DVM, PhD), Hilana dos Santos Sena Brunel¹ (DVM,
PhD), Paulo Henrique Sampaio da Silva²(DVM),
Cecília Ferreira Amaral Silva(DVM), André Rodrigues da Cunha Barreto
Vianna³(DVM, PhD), Eduardo Maurício Mendes de Lima²*(DVM, PhD).
¹Biocell Lab - Cellular Therapy, SMPW Qd.05,
Brasília, DF 71735-500. ²Department of Veterinary Anatomy, Faculty of Agronomy
and Veterinary Medicine, University of
Brasília, ³Department of Veterinary Anatomy, Federal University of
Paraná-Palotina Sector, Brazil. *Corresponding Author:limaemm@hotmail.com
ABSTRACT
Malard
PF, Santos Sena HB, Sampaio da Silva PH, Amaral Silva CF, Barreto Vianna AR,
Mendes de Lima EM., Mesenchymal stem cell treatment for
superficial digital flexor tendon injury in equine athletes,
Onl J Vet Res., 24 (3): 150-158, 2020. Athlete horses have a high incidence of tendon
injuries. We report use of mesenchymal stem cells (MSC’s) for tendonitis in 8 male and 2 female lame
quarter horse athletes diagnosed
with superficial digital flexor tendonitis. MSCs were isolated from adipose
tissue of a normal horse. In order to ensure cell purity and functional
potential, flow cytometry was used to identify CD44, CD90, CD105, OCT3 / 4 and
SOX2 proteins. The cells were injected with aid of ultrasonography into intra
and/or peri-lesional regions. Clinical and image evaluations were performed
before, at day 0 and 30 days after treatment. At day 30, we detected a 100%
reduction of anechoic ultrasound patterns in 7 horses and 75% in 3 horses with
corresponding increases in isoechoic patterns. Our results suggest that
mesenchymal stem cell treatments for superficial digital flexor tendon injury
in horses was beneficial but did not guarantee full recovery.
Keywords: cytometry, horses, MSC,
orthopaedics, tendinopathy.