Volume 23 (4):307-314, 2019.
PCR for detection of eggs and adult worms of Teladorsagia circumcincta in sheep.
Houida Hamle Abed, Mohammed Thabit Salih Al-Zubaidi
Department of Parasitology, College of Veterinary Medicine, University of Baghdad, Iraq
Abed HH, Al-Zubaidi MTS., PCR for detection of eggs and adult worms of Teladorsagia circumcincta in sheep., Onl J Vet Res., 23 (4):307-314, 2019. Current polymerase chain reactions (PCR’s) to detect Trichostrongylidae eggs in sheep are not practical for broad diagnostic application. We describe a PCR to detect eggs and adult worms of Teladorsagia circumcincta in fecal and abomasa samples. GenBank 28s and 18s sequences of T. circumcincta (HF571529.1) were used to design primers. We applied a 2nd internal transcribed spacer ribosomal DNA (ITS2) as a gene marker for molecular identification of Teladosragia (Ostertagia) species. To detect DNA in feces (n=50) we used an extraction kit but to detect DNA of Teladorsagia spp adult worms (n=120) we used a DNA tissue isolation kit. Sixty random specimens of Ostertagia spp. and 50 stool samples were used for PCR. By fecal flotation (n=50) we detected ~30 samples positive to Trichostrongylidae spp eggs but by PCR we identified ~20 Teladosragia circumcincta in feces and ~60 adult worms. Phylogenic analysis revealed 100% similarity with a Morocco isolate of Teladosragia circumcincta. We find that this PCR could accurately identify nematodes species.
Keywords: Teladosragia circumcincta, ITS2, PCR, Trichostrongylidae.