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OJBTM
Online Journal of Bioinformatics ©
Volume 12(2):255-273, 2011
Phylogenetic
analysis of
mitochondrial 16s rRNA and NADH Dehydrogenase
genes of Takifugu (Pisces: Tetraodontiformes:
Tetraodontidae)
M. Jagannath (MSc), K. Umadevi (PhD) * and Subhalaxmi
Nayak (MSc)
DBT – BIF
Programme, Department of Marine Living
Resources, Andhra
University, Visakhapatnam, India,
ABSTRACT
Jagannath M, Umadevi K, Subhalaxmi
N., Phylogenetic
analysis of mitochondrial 16s rRNA and
NADH Dehydrogenase genes of Takifugu
(Pisces: Tetraodontiformes: Tetraodontidae),
Online J Bioinformatics,
12(2):255-273, 2011. Phylogenetic
analysis of mitochondrial 16s rRNA and
NADH Dehydrogenase (ND)
gene sequences of Takifugu (Pisces: Tetraodontiformes:
Tetraodontidae) was performed.
Mitochondrial 16s rRNA
genes and orthologous mitochondrial ND
gene subunits
sequences were extracted from GenBank and
multiple
sequence alignment was performed using ClustalX.
The phylogenetic tree was constructed by
maximum parsimony with
PHYLIP (Phylogeny Inference Package).
Results showed that
ND IV gene was the primitive ancestor in Takifugu genus. Each group of
the
phylogram showed
+90% identity suggesting that the
sequences are highly conserved. Takifugu snyderi and Takifugu stictonotus, Takifugu rubripes and Takifugu chinensis,
Takifugu obscurus and Takifugu fasciatus,
Takifugu niphobles and
Takifugu pardalis, Takifugu poecilonotus
and Takifugu exascurus
were closely associated
Keywords:
Phylogeny, PHYLIP, 16s rRNA, NADH Dehydrogenase,
Takifugu.