Effect of Dimethyl sulfoxide on clinical signs and acute phase proteins during endotoxemia induced by Escherichia Coli Serotype O55:B5 in sheep.

MAIN

©1996-2018. All Rights Reserved. Online Journal of Veterinary Research . You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.

OJVRTM

Online Journal of Veterinary Research©

Volume 18(3):188-200, 2014. Redacted 2017.

Effect of Dimethyl sulfoxide on clinical signs and acute phase proteins during endotoxemia

induced by Escherichia Coli Serotype O55:B5 in sheep.

Amir Saeed Samimi¹,Ali Hajimohammadi¹, Mehdi Fazeli², Saeed Nazifi¹

¹Department(s) of Clinical Studies, ²Basic Sciences, School of Veterinary Medicine; Shiraz University, Shiraz, Iran

ABSTRACT

Samimi AS, Hajimohammadi A, Fazeli M, Nazifi S., Effect of Dimethyl sulfoxide on clinical signs and acute phase proteins during endotoxemia induced by Escherichia Coli Serotype O55:B5 in sheep, Onl J Vet Res., 18(3):188-200, 2014. The effect of Dimethyl sulfoxide (DMSO) on endotoxemia induced by IV infusion of Escherichia coli serotype O55:B5 endotoxin in fat-tailed sheep is described, Groups of 5 each 1yr old rams were administered 2 μg/kg li serotype O55:B5 intravenously (IV) and then given IV fluid therapy for 1.5 hours. Three hours after endotoxin infusion 1 g/kg DMSO was given to 1 group and 2 g/kg to another group along with the IV fluid therapy for 60 min. Five controls were given IV fluid after endotoxin infusion or another 5 sheep, IV fluid without drug. Clinical signs were recorded before and after treatments and electrocardiograms (ECG) to evaluate arrhythmias. Blood was collected at 0, 1.5, 3, 4.5 and 24 hours post-endotoxin injection to determine changes in acute phase proteins (APPs), haptoglobin (Hp), serum amyloid A (SAA), fibrinogen (Fib), and ceruloplasmin (Cp) concentrations. Compared with controls, APPs increased sharply after endotoxemia infusion in treated sheep but after DMSO administration serum APPs declined. No difference occurred with APPs levels between treated groups except with SAA levels which were lower in animals given the lower dose of DMSO. Increases in APPs levels occurred prior to appearance of clinical signs. Results suggest that DMSO reduced acute clinical signs and acute phase response protein after endotoxemia induced by E coli serotype O55:B5 in rams.

Keywords: Endotoxemia, Acute phase proteins, Dimethyl sulfoxide, Iranian fat-tailed sheep.

MAIN

FULL-TEXT(SUBSCRIPTION OR PURCHASE TITLE $25USD)