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Online Journal of Veterinary Research


Volume 26 (7): 508-518, 2022.

Canine periodontal ligament and bone marrow mesenchymal stem cell CD34 and CD44 markers.


Hamideh Salari Sedigh1,2 DVM DVSc, Shahram Jamshidi3 DVM DVSc, Anna Saffarpour4 DDS,

Mahdi Ashouri5 DDS, Seyed Mahdi Nassiri6 DVM PhD.


1-Department of Clinical Science, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, 2-Oral & Maxillofacial Diseases Research Center, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, 3- Department of clinical Sciences, Faculty of Veterinary Medicine,University of Tehran, 4- Department of Periodontology, Tehran University of Medical Sciences, International Campus, 5- Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Shahed University of Medical Sciences, Tehran 6- Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.




Sedigh HS, Jamshidi S, Saffarpour A, Ashouri M, Nassiri SM., Canine periodontal ligament and bone marrow mesenchymal stem cell CD34 and CD44 markers, Onl J Vet Res., Volume 26 (7): 508-518, 2022. Periodontal disease causes destruction of connective tissue that retains teeth within the jaw. We report expression of periodontal ligament and bone marrow stem cell-associated surface markers CD34 and CD44. By RT-PCR we found that genes for expression of alkaline phosphatase, periostin, osteocalcin and S100A4 genes were higher in periodontal ligament stem cells. Both mesenchymal stem cells differentiated into osteoblasts and adipocytes. Scanning and transmission electron microscopy revealed extracellular collagen fibers generated by ligament stem cells. Findings suggest undifferentiated mesenchymal periodontal ligament stem cells may regenerative dental structures in dogs.


Keywords: Canine, periodontal ligament, bone marrow, stem cells, CD44 CD34 markersd, differentiation.