1994-2020. All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors.


Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X


Volume 22 (11):1021-1028, 2018.

Effect of platelet rich plasma on lamb spermatogonia In vitro stem cell growth.

Nooshin Kazemi, Peyman Rahimi-Feyli, Ali-Asghar Moghaddam


Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.




Kazemi N, Rahimi-Feyli P, Moghaddam AA., Effect of platelet rich plasma on lamb spermatogonia In vitro stem cell growt, Onl J Vet Res., 22 (11):1021-1028, 2018. Spermatogonial stem cells (SSC) provide an excellent model system for differentiation, development and functioning of testes and for production of transgenic animals. We describe effects of different concentrations of Platelet-Rich Plasma (PRP) on SSC colony formation over 10 days incubation. Testicular cells were isolated from testes of prepubertal lambs by two-step enzymatic digestion then cultured for 10 days. Control cells were grown in basic DMEM media with 1% antibiotic and 5% FBS. Treated cells were grown in basic media with 1%, 2% or 4% PRP. Culture media was changed every 72h and SSC colony formation was monitored by inverted microscope. SSCs were identified by immunocytochemistry assay against PGP9.5. SSC numbers increased in all samples (P < 0.05) at day 10 compared with days 7 and 4 but treatments with 4% PRP decreased SSC colonies and surface area. We find that PRP had no significant effect on growth of SSCs in Vitro.



Key Words: Stem cell, Spermatogonia, Colony formation, Platelet-Rich Plasma.