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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 22 (11):1021-1028, 2018.
Effect of platelet rich plasma on lamb spermatogonia In vitro stem cell growth.
Nooshin Kazemi, Peyman Rahimi-Feyli, Ali-Asghar Moghaddam
Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.
Kazemi N, Rahimi-Feyli P, Moghaddam AA., Effect of platelet rich plasma on lamb spermatogonia In vitro stem cell growt, Onl J Vet Res., 22 (11):1021-1028, 2018. Spermatogonial stem cells (SSC) provide an excellent model system for differentiation, development and functioning of testes and for production of transgenic animals. We describe effects of different concentrations of Platelet-Rich Plasma (PRP) on SSC colony formation over 10 days incubation. Testicular cells were isolated from testes of prepubertal lambs by two-step enzymatic digestion then cultured for 10 days. Control cells were grown in basic DMEM media with 1% antibiotic and 5% FBS. Treated cells were grown in basic media with 1%, 2% or 4% PRP. Culture media was changed every 72h and SSC colony formation was monitored by inverted microscope. SSCs were identified by immunocytochemistry assay against PGP9.5. SSC numbers increased in all samples (P < 0.05) at day 10 compared with days 7 and 4 but treatments with 4% PRP decreased SSC colonies and surface area. We find that PRP had no significant effect on growth of SSCs in Vitro.
Key Words: Stem cell, Spermatogonia, Colony formation, Platelet-Rich Plasma.