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Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X


Volume 26 (10): 770-776, 2022.

Diagnosis of equine and bovine cutaneous melanoma and squamous cell carcinoma

by staining and immunohistochemistry.


Farhang Sasani, Javad Javanbakht, Mehdi Aghamohammad Hassan, Farajollaheh Adibhashemi, Soheila Hemmati.


Faculty of Veterinary Medicine, Tehran University, Iran.




Sasani F, Javanbakht J, Hassan MA, Adibhashemi F, Hemmati S., Diagnosis of equine and bovine cutaneous melanoma and squamous cell carcinoma by staining and immunohistochemistry, Onl J Vet Res., 26 (10): 770-776, 2022. We report diagnoses for cutaneous and squamous cell carcinomas from skin of horses and cattle by hematoxylin-eosin, Fontana-Masson, melanin bleach, S-100 immunochemistry and HMB45 antigen stains. Serial 5m-thick sections were cut from routine formalin-fixed paraffin blocks of squamous cell carcinomas and melanomas from 6 horses and by hematoxylin-eosin stain. S-100 protein and HMB45 tests confirmed 6 of 9 tumors whereas 3 were doubtful. By Fontana-Masson silver and melanin bleaching methods all tumors tested positive. However for squamous cell carcinoma the 3 tests were negative. Diagnosis of suspicious melanoma tumor cases should be confirmed and reconfirmed by staining and immunohistochemistry.


Key words: Melanoma, Squamous Cell Carcinoma, HMB45 antigen, S-100 protein.