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Online Journal of Veterinary Research


Volume (10):961-976, 2019

Effect of Spinacia oleracea leaf extract on hypomagnesemia markers in female Wistar rats


Mustafa Majid Obaid and Luma Waleed Khaleel.


Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Baghdad.



Obaid MM, Khaleel LW., Effect of Spinacia oleracea leaf extract on hypomagnesemia markers in female Wistar rats, Onl J Vet Res., (10):961-976, 2019. Four groups of 10 rats each were gavaged 1.39mg/kg Potassium chloride (KCL) daily for 56 days (1), KCL with 250mg/kg spinach (Spinacia oleracea) (SO) extract (2), 250mg/kg SO (3) or KCL with 0.1mg Magnesium sulphate (MSO) (4). Ten controls were gavaged distilled water. Fasting cardiac blood was aspirated at 56 day for magnesium (Mg+2), potassium (K+2), calcium (Ca+2), lactate dehydrogenase (LDH) and troponin-1. At sacrifice we excised heart and bone tissue for microscopy. Compared with controls, in rats gavaged KCL, we found large declines (P <0.005) in Mg+2 (~-35%) and Ca+2 (~-51%) and increases in LDH (26%), K+2 (~16%) and troponin (~82%) (P < 0.05). In those given KCL with extract we found no significant variation. In rats given only extract we found slight increases in Mg and falls in Ca. In rats given KCL we observed hemorrhages, edema and necrosis in myocardiac muscle fibers. We also observed congestion in rats given KCL with extract but found no lesions in those given only extract. In hypo-magnesemic rats we observed longitudinal cracks in bone.


Key Words: Potassium chloride, Toxicity, Spinacea, rats. Raw data provided.