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OJVRTM
Online Journal of Veterinary Research ©
(Including
Medical and Laboratory Research)
Established
1994
ISSN
1328-925X
Volume
27 (12): 659-670,
2023
Growth of sheep spermatogonial stem cells in colony stimulating and glial
cell line-derived neurotrophic factors.
Peyman Rahimi-Feyli1, Aliasghar Moghaddam1, Parviz
Tajik2, Shiva Shafiei2, Zahra Nikousefat1,
Mojtaba Goli1, Nafiseh Alighazi2, Reza Narenji-Sani3
1Department (s) clinical Sciences, Faculty’s
Veterinary Medicine, Razi University, Kermanshah, 2Theriogenology,
University of Tehran, 3Clinical Sciences, Semnan University, Iran.
ABSTRACT
Rahimi-Feyli P, Moghaddam A, Tajik P, Shafiei
S, Nikousefat Z, Goli M, Alighazi N, Narenji-Sani R.,
Growth of sheep spermatogonial stem cells in colony
stimulating and glial cell line-derived neurotrophic factors, Onl J Vet Res., 27 (12): 659-670, 2023. We report In vitro colony formation of spermatogonia stem cells (SSC) from prepubertal
lambs in glial cell line-derived
neurotrophic factor (GDNF) and cerebrospinal
fluid 1 (CSF1). Testes cells were isolated by two-step
enzymatic digestion for differential plating incubated in 40ng/ml GDNF),
40ng/ml CSF1 with 40ng/ml GDNF) or 40ng/ml CSF1). Controls were not
treated. Colonies were assayed at 4, 7 and 10 days cultivation by
immunocytochemistry against PGP9.5 and ultrastructure by light and transmission
electron microscopy. Compared with day 4, stem cell colonies had grown 6.8 in
controls, 39% in CSF, 29 in GDNF and 48% in both (P < 0.05). Surface area of
all colonies increased 48-52% (P<0.05). We find that GDNF with CSF1 may
enhance growth of prepubertal sheep stem cells.
Key words: Spermatogonial stem cell, CSF1, GDNF, Sheep.
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