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Online Journal of Veterinary Research © 

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

Volume 27 (12): 659-670, 2023

Growth of sheep spermatogonial stem cells in colony stimulating and glial cell line-derived neurotrophic factors.


Peyman Rahimi-Feyli1, Aliasghar Moghaddam1, Parviz Tajik2, Shiva Shafiei2, Zahra Nikousefat1,

Mojtaba Goli1, Nafiseh Alighazi2, Reza Narenji-Sani3


1Department (s) clinical Sciences, Faculty’s Veterinary Medicine, Razi University, Kermanshah, 2Theriogenology, University of Tehran, 3Clinical Sciences, Semnan University, Iran.




Rahimi-Feyli P, Moghaddam A, Tajik P, Shafiei S, Nikousefat Z, Goli M, Alighazi N, Narenji-Sani R., Growth of sheep spermatogonial stem cells in colony stimulating and glial cell line-derived neurotrophic factors, Onl J Vet Res., 27 (12): 659-670, 2023. We report In vitro colony formation of spermatogonia stem cells (SSC) from prepubertal lambs in glial cell line-derived neurotrophic factor (GDNF) and cerebrospinal fluid 1 (CSF1). Testes cells were isolated by two-step enzymatic digestion for differential plating incubated in 40ng/ml GDNF), 40ng/ml CSF1 with 40ng/ml GDNF) or 40ng/ml CSF1). Controls were not treated. Colonies were assayed at 4, 7 and 10 days cultivation by immunocytochemistry against PGP9.5 and ultrastructure by light and transmission electron microscopy. Compared with day 4, stem cell colonies had grown 6.8 in controls, 39% in CSF, 29 in GDNF and 48% in both (P < 0.05). Surface area of all colonies increased 48-52% (P<0.05). We find that GDNF with CSF1 may enhance growth of prepubertal sheep stem cells.


Key words: Spermatogonial stem cell, CSF1, GDNF, Sheep.