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Online Journal of Veterinary Research©
Volume 23 (5):425-430, 2019.
†Effect of gonadotropin releasing hormone on caprine spermatogonial stem cell proliferation.
Yasaman Gholampour1, Peyman Rahimi-Feyli1, Aliasghar Moghaddam1, Samad Alimohammadi2
1- Department of clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, 2- Department of Basic Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.
Gholampour Y, Rahimi-Feyli P,† Moghaddam A, Alimohammadi S., Effect of gonadotropin releasing hormone on caprine spermatogonial stem cell proliferation, Onl J Vet Res., 23 (5):414-430, 2019. We report dose related effect of Gonadotropin Releasing Hormone (GnRH) on 10 day culture of caprine spermatogonial stem cells (SSC) in presence of sertoli cells. SSCs were isolated from testes of prepubertal goat by two step enzymatic digestions and then treated 10 days in media culture with 0/5, 1, 2 or 4μg/ml GnRH. SSCís were identified by immunocytochemistry against PGP9.5. Controls were DMEM media with 1% antibiotic and 5% FBS but no GnRH. Media was changed every 72h and colony number and diameter measured at 4, 7 and 10 days. Numbers and diameters of colonies were counted on inverted microscope and surface area by image J software every 3 days. Colonies increased (P < 0.05) ~16% by day 4 and ~40 by day 10. Compared with controls we found ~11% increase in media treated with 1ug/ml GnRH but a decline (P < 0.05) of 40% in 4ug/ml. Similar results were found with colony surface areas. Our results suggest that 4 μg/ml GnRH may inhibit growth of goat spermatogonial stem cells.
Key Words: Spermatogonial Stem Cell, Sertoli, Caprine, GnRH. Raw data provided.